Spt6 is required for the fidelity of promoter selection
Spt6 is required for the fidelity of promoter selection
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DOI：
10.1101/347575
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
Stephen M. Doris;James Chuang;James Chuang;O. Viktorovskaya;M. Murawska;Dan Spatt;L. S. Churchman;F. Winston
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文献类型：
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作者：
Stephen M. Doris;James Chuang;James Chuang;O. Viktorovskaya;M. Murawska;Dan Spatt;L. S. Churchman;F. Winston
Spt6是一个保守的因子，控制整个基因组的转录和染色质结构。尽管Spt6被认为是一种延伸因子，但酿酒酵母中的Spt6突变允许编码区域内转录本水平升高，这表明Spt6也控制起始。为了解决转录和染色质结构对Spt6的要求，我们结合了四种全基因组方法。我们的研究结果表明，Spt6在数千个基因内启动子上抑制转录起始。我们对这些基因内启动子进行了表征，并发现与基因启动子保守的序列特征。最后，我们发现Spt6也调节大多数基因启动子的转录起始，并提出了一个起始位点竞争模型来解释这一点。总之，我们的研究结果表明，Spt6控制着整个基因组转录起始的保真度，并揭示了通过基因内启动子表达替代遗传信息的潜力。
Spt6 is a conserved factor that controls transcription and chromatin structure across the genome. Although Spt6 is viewed as an elongation factor, spt6 mutations in Saccharomyces cerevisiae allow elevated levels of transcripts from within coding regions, suggesting that Spt6 also controls initiation. To address the requirements for Spt6 in transcription and chromatin structure, we have combined four genome-wide approaches. Our results demonstrate that Spt6 represses transcription initiation at thousands of intragenic promoters. We characterize these intragenic promoters, and find sequence features conserved with genic promoters. Finally, we show that Spt6 also regulates transcription initiation at most genic promoters and propose a model of initiation-site competition to account for this. Together, our results demonstrate that Spt6 controls the fidelity of transcription initiation throughout the genome and reveal the magnitude of the potential for expressing alternative genetic information via intragenic promoters.