Complete genome sequencing and phylogenetic analysis of dengue type 1 virus isolated from Jeddah, Saudi Arabia.
Complete genome sequencing and phylogenetic analysis of dengue type 1 virus isolated from Jeddah, Saudi Arabia.
复制标题
从沙特阿拉伯吉达分离的1型登革热1型病毒的完整基因组测序和系统发育分析。
DOI：
10.1186/s12985-014-0235-7
复制
发表时间：
2015-01-16
期刊：
影响因子：
4.8
通讯作者：
Jamjoom G
中科院分区：
文献类型：
--
作者：
Azhar EI;Hashem AM;El-Kafrawy SA;Abol-Ela S;Abd-Alla AM;Sohrab SS;Farraj SA;Othman NA;Ben-Helaby HG;Ashshi A;Madani TA;Jamjoom G
登革热病毒（DENV）是蚊媒病毒，可引起从轻度发烧到严重登革热感染的疾病。这些病毒在几个热带和亚热带地区流行。自1994年以来，沙特阿拉伯西部地区已报告了DENV血清型1、2和3（DENV-1、DENV-2和DENV-3）的多次爆发。来自至少两种DENV-1基因型（亚洲和美洲/非洲基因型）的菌株一直在沙特阿拉伯西部传播，直到2006年。然而，沙特阿拉伯报道的所有先前研究都是基于包膜（E）基因的部分测序数据，而没有任何关于在沙特阿拉伯流行的任何DENV血清型的全基因组序列的报道。在这里，我们报告了2011年从沙特阿拉伯吉达的一名患者中分离的DENV-1毒株（DENV-1-Jeddah-1-2011）的分离和第一个完整的基因组序列。DENV-1-Jeddah-1-2011株与1998年吉布提分离株D1/H/IMTSSA/98/606（亚洲基因型）同源性较高。对完整包膜基因的进一步分析显示，DENV-1-Jeddah-1-2011毒株与2004-2006年吉达报告的分离株以及最近来自索马里的分离株之间存在密切关系，这表明亚洲基因型在该地区广泛存在。这些数据表明，属于亚洲基因型的菌株可能在2004年之前很久就被引入沙特阿拉伯，最有可能是由非洲朝圣者引入的，并至少在2011年之前继续在沙特阿拉伯西部传播。最重要的是，这些结果表明，来自登革热流行地区的朝圣者可以在沙特阿拉伯和世界其他地区的新DENV传播中发挥重要作用。因此，全基因组序列的可用性将作为未来DENV-1病毒流行病学研究的参考。
Dengue viruses (DENVs) are mosquito-borne viruses which can cause disease ranging from mild fever to severe dengue infection. These viruses are endemic in several tropical and subtropical regions. Multiple outbreaks of DENV serotypes 1, 2 and 3 (DENV-1, DENV-2 and DENV-3) have been reported from the western region in Saudi Arabia since 1994. Strains from at least two genotypes of DENV-1 (Asia and America/Africa genotypes) have been circulating in western Saudi Arabia until 2006. However, all previous studies reported from Saudi Arabia were based on partial sequencing data of the envelope (E) gene without any reports of full genome sequences for any DENV serotypes circulating in Saudi Arabia. Here, we report the isolation and the first complete genome sequence of a DENV-1 strain (DENV-1-Jeddah-1-2011) isolated from a patient from Jeddah, Saudi Arabia in 2011. Whole genome sequence alignment and phylogenetic analysis showed high similarity between DENV-1-Jeddah-1-2011 strain and D1/H/IMTSSA/98/606 isolate (Asian genotype) reported from Djibouti in 1998. Further analysis of the full envelope gene revealed a close relationship between DENV-1-Jeddah-1-2011 strain and isolates reported between 2004–2006 from Jeddah as well as recent isolates from Somalia, suggesting the widespread of the Asian genotype in this region. These data suggest that strains belonging to the Asian genotype might have been introduced into Saudi Arabia long before 2004 most probably by African pilgrims and continued to circulate in western Saudi Arabia at least until 2011. Most importantly, these results indicate that pilgrims from dengue endemic regions can play an important role in the spread of new DENVs in Saudi Arabia and the rest of the world. Therefore, availability of complete genome sequences would serve as a reference for future epidemiological studies of DENV-1 viruses.