A 45 kDa protein related to PPARγ2, induced by peroxisome proliferators, is located in the mitochondrial matrix
A 45 kDa protein related to PPARγ2, induced by peroxisome proliferators, is located in the mitochondrial matrix
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DOI：
10.1016/s0014-5793(00)01814-7
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发表时间：
2000-07-28
期刊：
影响因子：
3.5
通讯作者：
Dauça, M
中科院分区：
文献类型：
--
作者：
Casas, F;Domenjoud, L;Dauça, M
除了通过激活几种过氧化物酶体增殖物激活受体（PPARs）参与控制核基因表达外，过氧化物酶体增殖物还影响线粒体活性。通过与线粒体T3受体（p43）的先前表征相类比，我们在细胞器中寻找过氧化物酶体增殖物靶的存在。使用针对PPARs不同结构域的几种抗血清，我们通过Western印迹、免疫沉淀和电子显微镜实验证明，与PPAR γ 2（mt-PPAR）相关的45 kDa蛋白位于大鼠肝线粒体基质中。此外，我们发现，通过氯贝特治疗的mt-PPAR的量增加。此外，在EMSA实验中，mt-PPAR通过与p43形成复合物而与位于线粒体D环中的DR 2序列结合。最后，对组织特异性表达的研究表明，在除脑以外的所有测试组织的线粒体中检测到mt-PPAR，其量与p43丰度呈正相关，（C）2000 Federation of European Biochemical Societies。由Elsevier Science B. V.出版，版权所有。
Besides their involvement in the control of nuclear gene expression by activating several peroxisome proliferator-activated receptors (PPARs), peroxisome proliferators influence mitochondrial activity. By analogy with the previous characterization of a mitochondrial T3 receptor (p43), we searched for the presence of a peroxisome proliferator target in the organelle, Using several antisera raised against different domains of PPARs, we demonstrated by Western blotting, immunoprecipitation and electron microscopy experiments, that a 45 kDa protein related to PPAR gamma 2 (mt-PPAR) is located in the matrix of rat liver mitochondria. In addition, we found that the amounts of mt-PPAR are increased by clofibrate treatment. Moreover, in EMSA experiments mt-PPAR bound to a DR2 sequence located in the mitochondrial D-loop, by forming a complex with p43. Last, studies of tissue-specific expression indicated that mt-PPAR is detected in mitochondria of all tissues tested except the brain in amounts positively related to p43 abundance, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.