Urine metabolic profiling of dementia rats with vital energy deficiency using ultra-high-performance liquid chromatography coupled with an orbitrap mass spectrometer
Urine metabolic profiling of dementia rats with vital energy deficiency using ultra-high-performance liquid chromatography coupled with an orbitrap mass spectrometer
复制标题
使用超高效液相色谱结合轨道阱质谱仪对生命能量缺乏的痴呆大鼠进行尿液代谢分析
DOI：
10.1002/jssc.202100837
复制
发表时间：
2021-11-25
影响因子：
3.1
通讯作者：
Jin, Yongri
中科院分区：
文献类型：
--
作者：
Huang, Yu;Liu, Zhiqiang;Jin, Yongri
痴呆是一种慢性、多因素诱发的神经退行性疾病，多发生于体质虚弱、精气不足的老年人。但气虚与痴呆发生发展的关系尚不清楚。本研究采用d-半乳糖和AlCl 3腹腔注射结合力竭性游泳建立气虚型痴呆大鼠模型。通过行为学、海马组织病理学和生化指标、血清生化指标等观察气虚痴呆大鼠模型的变化。基于超高效液相色谱结合轨道阱质谱仪的尿液代谢组学也被用于发现内源性代谢谱和疾病相关的生物标志物，并探讨气虚痴呆的潜在机制。在确定的31种潜在生物标志物中，有9种涉及代谢途径。四种主要类型是苯丙氨酸、酪氨酸和色氨酸代谢，牛磺酸和亚牛磺酸代谢，柠檬酸循环和嘧啶代谢。气虚型痴呆的发病机制主要是神经毒素蓄积和机体衰老导致氧化应激损伤和神经保护物质的丧失。精气不足会抑制人体的能量代谢，最终导致痴呆症的加重。
Dementia is a chronic and multifactor-induced neurodegenerative disorder that occurs frequently in the elderly with weak constitution and insufficient vital energy. However, the relationship between vital energy deficiency and the occurrence and development of dementia is still unclear. In this study, a rat model of dementia with vital energy deficiency was established through intraperitoneal injection with d-galactose and AlCl3 and combined with exhaustive swimming. Changes in the dementia with vital energy deficiency rat model were assessed by examining behaviors, hippocampal histopathological and biochemical parameters, and serum biochemical parameters. Urine metabolomics based on ultra-high-performance liquid chromatography coupled with an orbitrap mass spectrometer was also used to discover endogenous metabolic profile and disease-related biomarkers and investigate the potential mechanism of dementia with vital energy deficiency. Among the 31 potential biomarkers that were identified, nine involved metabolic pathways. The four main types were phenylalanine, tyrosine and tryptophan metabolism, taurine and hypotaurine metabolism, and citrate cycle and pyrimidine metabolism. The pathogenesis of dementia with vital energy deficiency is mainly neurotoxin accumulation and body aging that leads to oxidative stress injury and loss of neuronal protective substances. Vital energy deficiency inhibits the body's energy metabolism and eventually leads to aggravate the dementia.