A HIGHLY SENSITIVE CYTOCHEMICAL BIOASSAY FOR PLASMA ANGIOTENSIN II
A HIGHLY SENSITIVE CYTOCHEMICAL BIOASSAY FOR PLASMA ANGIOTENSIN II
复制标题
血浆血管紧张素 II 的高灵敏细胞化学生物测定
DOI：
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发表时间：
1983
影响因子：
3.2
通讯作者：
J. Alaghband‐Zadeh
中科院分区：
文献类型：
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作者：
Julia C Jones;G. Macgregor;G. Sagnella;J. Morton;J. Alaghband‐Zadeh
建立了一种测定人血浆中血管紧张素II的高灵敏度细胞化学生物测定法。该测定依赖于血管紧张素II改变普鲁士蓝染色和显微密度法测量的肾小球带还原能力的能力。血管紧张素II浓度在0.05~5.0fmol/L范围内与染色强度呈负相关，血浆中该方法的灵敏度为50fmol/L，精密度指数为0.07±0.04(均值±SD；n=15)，质控样品的变异系数为34%。对血管紧张素II的反应是特异的；要产生类似的效果，需要大约102倍的血管紧张素III和大约106倍的ACTH。血管紧张素I无明显活性。在5名正常受试者中，钠摄入量与生物活性血管紧张素II之间存在显著的负相关关系。在无肾受试者中检测到极低水平的血管紧张素II。
A highly sensitive cytochemical bioassay has been developed for measuring angiotensin II in human plasma. The assay depends on the ability of angiotensin II to alter the reducing potency of the zona glomerulosa as measured by Prussian blue staining and microdensitometry. An inverse correlation between the intensity of the stain and the logarithm of concentration existed over the range 0.05‐5.0 fmol/l of angiotensin II. The limit of sensitivity of the assay in plasma was 50 fmol/l; the index of precision was 0.07 ± 0.04 (mean ± SD; n = 15); and the coefficient of variation of a quality control sample was 34%. The response was specific for angiotensin II; approximately 102 times more angiotensin III and approximately 106 times more ACTH was required to produce a similar effect. Angiotensin I had no significant activity. A significant inverse relationship existed between sodium intake and bioactive angiotensin II in 5 normal subjects studied on low, normal and high sodium diets. Extremely low levels of angiotensin II were detected in anephric subjects.