A Functional Variable Number of Tandem Repeats is Located at the 5' Flanking Region of the Human Secretin Gene Plays a Downregulatory Role in Expression
A Functional Variable Number of Tandem Repeats is Located at the 5' Flanking Region of the Human Secretin Gene Plays a Downregulatory Role in Expression
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DOI：
10.1007/s12031-008-9083-5
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发表时间：
2008-11-01
影响因子：
3.1
通讯作者：
Chow, Billy K. C.
中科院分区：
文献类型：
--
作者：
Lee, Leo T. O.;Lam, Ian P. Y.;Chow, Billy K. C.
促胰液素是一种在脑-肠轴中发挥多种功能的肽类激素。在本报告中，我们调查，通过启动子分析，可变的串联重复序列（VNTR）的潜在功能，位于5'上游区的人分泌素基因，我们首次证明，这种VNTR可以下调转录的人分泌素基因在启动子特异性的方式。发现VNTR在沉默启动子中的效率与存在于其中的重复单元的数目直接相关。我们还显示了76个中国人的脱氧核糖核酸序列以及VNTR的长度多态性。这些结果共同表明，VNTR可能是一个潜在的功能调节器，以控制不同个体的人分泌素基因的表达。
Secretin is a peptide hormone playing multiple functions in the brain-gut axis. In this report, we investigated, by promoter analysis, the potential function of the variable of tandem repeats (VNTR), located at the 5' upstream region of the human secretin gene, and we demonstrated for the first time that this VNTR could downregulate transcription of the human secretin gene in a promoter-specific manner. The efficiency of VNTR in silencing the promoter was found to be directly related the number of repetitive units residing within. We also showed the deoxyribonucleic acid sequence as well as the length polymorphism of the VNTR of 76 Chinese individuals. These results collectively suggest that VNTR could potentially be a functional regulator to control the expression of the human secretin gene in different individuals.