Water-Soluble Coenzyme Q10 Reduces Rotenone-Induced Mitochondrial Fission
Water-Soluble Coenzyme Q10 Reduces Rotenone-Induced Mitochondrial Fission
复制标题
水溶性辅酶 Q10 减少鱼藤酮诱导的线粒体裂变
DOI：
10.1007/s11064-016-2143-2
复制
发表时间：
2017-04-01
影响因子：
4.4
通讯作者：
Li, P. Andy
中科院分区：
文献类型：
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作者：
Li, Hai-Ning;Zimmerman, Mary;Li, P. Andy
帕金森病是一种神经退行性疾病，其特征是线粒体功能障碍和氧化应激。它通常伴有线粒体动力学失衡以及与功能受损相关的线粒体形态变化。本研究的目的是确定鱼藤酮（一种已知可模拟帕金森病病理生理学的药物）对线粒体动力学的影响。此外，本研究还探讨了水溶性辅酶Q10（CoQ10）对鱼藤酮诱导的小鼠神经元HT22细胞毒性的保护作用。我们的研究结果表明，鱼藤酮提高了线粒体分裂标志物动力相关蛋白1（Drp1）和线粒体分裂蛋白1（Fis1）的蛋白表达，并导致线粒体碎片化增加，这通过线粒体染色和形态分析得以证实。水溶性辅酶Q10通过将Drp1和Fis1蛋白表达降低至鱼藤酮处理前的水平，以及减少鱼藤酮治疗相关的线粒体碎片化，从而防止了线粒体动力学失衡。因此，水溶性辅酶Q10可能在治疗帕金森病患者方面具有治疗潜力。
Parkinson's disease is a neurodegenerative disorder characterized by mitochondrial dysfunction and oxidative stress. It is usually accompanied by an imbalance in mitochondrial dynamics and changes in mitochondrial morphology that are associated with impaired function. The objectives of this study were to identify the effects of rotenone, a drug known to mimic the pathophysiology of Parkinson's disease, on mitochondrial dynamics. Additionally, this study explored the protective effects of watersoluble Coenzyme Q10 (CoQ10) against rotenone-induced cytotoxicity in murine neuronal HT22 cells. Our results demonstrate that rotenone elevates protein expression of mitochondrial fission markers, Drp1 and Fis1, and causes an increase in mitochondrial fragmentation as evidenced through mitochondrial staining and morphological analysis. Water-soluble CoQ10 prevented mitochondrial dynamic imbalance by reducing Drp1 and Fis1 protein expression to pre-rotenone levels, as well as reducing rotenone treatmentassociated mitochondrial fragmentation. Hence, watersoluble CoQ10 may have therapeutic potential in treating patients with Parkinson's disease.