Long-term effects of knitted silk-collagen sponge scaffold on anterior cruciate ligament reconstruction and osteoarthritis prevention
Long-term effects of knitted silk-collagen sponge scaffold on anterior cruciate ligament reconstruction and osteoarthritis prevention
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针织丝胶原海绵支架对前交叉韧带重建和预防骨关节炎的远期效果
DOI：
10.1016/j.biomaterials.2014.06.019
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发表时间：
2014-09-01
期刊：
影响因子：
14
通讯作者：
Ouyang, Hong-Wei
中科院分区：
文献类型：
--
作者：
Shen, Weiliang;Chen, Xiao;Ouyang, Hong-Wei
前交叉韧带（ACL）损伤后由于关节内流体环境的动态变化而难以愈合。以前，我们已经取得了令人满意的再生皮下肌腱/韧带与针织丝胶原海绵支架由于其特定的“内部空间保存”的属性。本研究旨在探讨针织蚕丝-胶原海绵支架对ACL再生和骨关节炎预防的长期影响。制备蚕丝-胶原海绵编织支架并植入兔前交叉韧带损伤模型。发现编织的丝-胶原海绵支架在手术后2个月增强梭形细胞向支架中的迁移和粘附。6个月后，用针织丝胶原海绵支架处理的ACL表现出韧带基因表达增加和更好的显微结构形态。18个月后，针织丝胶原海绵支架治疗组具有更成熟的韧带结构和直接的韧带-骨愈合。与皮下植入相比，植入的针织丝胶原海绵支架降解慢得多。此外，编织的丝绸-胶原蛋白海绵支架有效地保护了关节表面软骨，并在术后长达18个月的时间内保留了关节空间。因此，这些研究结果表明，针织丝-胶原海绵支架可以再生功能性ACL并长期预防骨关节炎，表明其作为ACL重建的功能性生物支架的临床用途。(C)2014爱思唯尔有限公司版权所有。
Anterior cruciate ligament (ACL) is difficult to heal after injury due to the dynamic fluid environment of joint. Previously, we have achieved satisfactory regeneration of subcutaneous tendon/ligament with knitted silk-collagen sponge scaffold due to its specific "internal-space-preservation" property. This study aims to investigate the long-term effects of knitted silk-collagen sponge scaffold on ACL regeneration and osteoarthritis prevention. The knitted silk-collagen sponge scaffold was fabricated and implanted into a rabbit ACL injury model. The knitted silk-collagen sponge scaffold was found to enhance migration and adhesion of spindle-shaped cells into the scaffold at 2 months post-surgery. After 6 months, ACL treated with the knitted silk-collagen sponge scaffold exhibited increased expression of ligament genes and better microstructural morphology. After 18 months, the knitted silk-collagen sponge scaffold-treated group had more mature ligament structure and direct ligament-to-bone healing. Implanted knitted silk-collagen sponge scaffolds degraded much more slowly compared to subcutaneous implantation. Furthermore, the knitted silk-collagen sponge scaffold effectively protected joint surface cartilage and preserved joint space for up to 18 months post-surgery. These findings thus demonstrated that the knitted silk-collagen sponge scaffold can regenerate functional ACL and prevent osteoarthritis in the long-term, suggesting its clinical use as a functional bioscaffold for ACL reconstruction. (C) 2014 Elsevier Ltd. All rights reserved.