SEWAGE COLIPHAGES STUDIED BY ELECTRON-MICROSCOPY
SEWAGE COLIPHAGES STUDIED BY ELECTRON-MICROSCOPY
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DOI：
10.1128/aem.45.3.1049-1059.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
NGUYEN, TM
中科院分区：
文献类型：
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作者：
ACKERMANN, HW;NGUYEN, TM
用35株大肠杆菌菌株对[来自加拿大魁北克省魁北克市]的污水进行富集，并用电镜对富集培养物的沉积物进行了研究。它们含有多达10种形态不同的颗粒。14份样品中t -even型噬菌体占主导地位。共富集了13个噬菌体，分别为肌病毒科(7)、茎状病毒科(2)、足病毒科(3)和微病毒科(1)。其中12个与已知的肠杆菌噬菌体种类对应，分别为121、K19、FC3-9、01、9266、T2、16-19、chi。度量。4、N4、T7和.vphi. x174。未检出立方RNA噬菌体和丝状噬菌体。121型和9266型以前只在罗马尼亚和南非观察到。通过形态学识别通常很简单。我们的调查技术是定性的，不能检测到所有存在的噬菌体。大多数富集菌株是多价的，噬菌体鉴定总是需要EM。一般来说，EM似乎是研究噬菌体地理学和生态学的首选方法。
Sewage [obtained from Quebec City, Quebec, Canada] was enriched with 35 Escherichia coli strains and sediments of enrichment cultures were studied by EM. They contained up to 10 varieties of morphologically different particles. T-even-type phages predominated in 14 samples. Thirteen phages were enriched, representing the families Myoviridae (7), Styloviridae (2), Podoviridae (3) and Microviridae (1). Twelve of these corresponded to known enterobacterial phage species, i.e., 121, K19, FC3-9, O1, 9266, T2, 16-19, .chi., .beta.4, N4, T7 and .vphi.X174. Cubic RNA phages and filamentous phages were not detected. Types 121 and 9266 were previously observed only in Romania and South Africa. Identification by morphology is usually simple. Our investigative technique is qualitative and will not detect all phages present. Most enrichment strains are polyvalent and EM is always required for phage identification. Generally, EM seems to be the method of choice for investigation of phage geography and ecology.