Cytotoxic T cell responses to haptenated cells III. Isolation and specificity analysis of continuously growing clones
Cytotoxic T cell responses to haptenated cells III. Isolation and specificity analysis of continuously growing clones
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细胞毒性 T 细胞对半抗原细胞的反应 III．
DOI：
10.1002/eji.1830101106
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发表时间：
1980
影响因子：
5.4
通讯作者：
H. von Boehmer
中科院分区：
文献类型：
--
作者：
W. Haas;Jacqueline Mathur‐Rochat;H. Pohlit;M. Nabholz;H. von Boehmer
使用各种程序从原代培养物中衍生出持续生长的细胞毒性 T 淋巴细胞 (CTL) 克隆，该原代培养物含有来自免疫小鼠的应答细胞和 3-(对磺基苯基重氮)-4-羟基苯乙酸 (SP) 或异硫氰酸荧光素 (FL) 偶联刺激细胞。 CTL 似乎必须经历一些变化（可能是遗传变化），才能在没有任何刺激物或填充细胞的情况下在 T 细胞生长因子条件培养基中持续生长。产生具有不同特异性的CTL克隆的最方便和可靠的程序是从在用T细胞生长因子调节的培养基中持续生长的原代培养细胞群的几个等分试样中建立。具有不同特异性的克隆在不同群体中分离。描述了仅限于 H-2Kk 和 H-2Dd 的 SP 和 FL 特异性 CTL 克隆，以及两个没有明显 H-2 限制的 FL 特异性 CTL 克隆。
Various procedures were used to derive continuously growing cytotoxic T lymphocyte (CTL) clones from a primary culture containing responder cells from immunized mice and 3‐(p‐sulfophenyldiazo)‐4‐hydroxylphenyl acetic acid (SP)‐or fluorescein isothiocyanate (FL)‐coupled stimulator cells. It seems likely that CTL have to undergo some change, possibly genetic, to be able to grow continuously in T cell growth factor conditioned medium in the absence of any stimulator or filler cells. The most convenient and reliable procedure to generate CTL clones with different specificities was to establish from several aliquots of a primary culture cell populations continuously growing in medium conditioned with T cell growth factor(s). Clones with different specificities segregated in the different populations. SP‐and FL‐specific CTL clones restricted to H‐2Kk, and H‐2Dd and two FL‐specific CTL clones with no apparent H‐2 restriction are described.