Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression.
Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression.
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PRP8 和 PRP17/CDC40 这两个酵母基因之间存在广泛的遗传相互作用，这两个基因参与前 mRNA 剪接和细胞周期进程。
作者：
S. Ben;C. Russell;I. Dix;J. Beggs;M. Kupiec
Biochemical and genetic experiments have shown that the PRP17 gene of the yeast Saccharomyces cerevisiae encodes a protein that plays a role during the second catalytic step of the splicing reaction. It was found recently that PRP17 is identical to the cell division cycle CDC40 gene. cdc40 mutants arrest at the restrictive temperature after the completion of DNA replication. Although the PRP17/CDC40 gene product is essential only at elevated temperatures, splicing intermediates accumulate in prp17 mutants even at the permissive temperature. In this report we describe extensive genetic interactions between PRP17/CDC40 and the PRP8 gene. PRP8 encodes a highly conserved U5 snRNP protein required for spliceosome assembly and for both catalytic steps of the splicing reaction. We show that mutations in the PRP8 gene are able to suppress the temperature-sensitive growth phenotype and the splicing defect conferred by the absence of the Prp17 protein. In addition, these mutations are capable of suppressing certain alterations in the conserved PyAG trinucleotide at the 3' splice junction, as detected by an ACT1-CUP1 splicing reporter system. Moreover, other PRP8 alleles exhibit synthetic lethality with the absence of Prp17p and show a reduced ability to splice an intron bearing an altered 3' splice junction. On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction.
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前 mRNA 剪接的第二个催化步骤。
DOI：
--
发表时间：
1995
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Umen,JG;Guthrie,C
通讯作者：
Guthrie,C
酵母 PRP8 剪接蛋白的哺乳动物类似物存在于 U4/5/6 小核核糖核蛋白颗粒和剪接体中。
DOI：
10.1073/pnas.86.22.8742
发表时间：
1989
影响因子：
11.1
作者：
Pinto,AL;Steitz,JA
通讯作者：
Steitz,JA
在前 mRNA 剪接的第二个催化步骤中，Prp16p、Slu7p 和 Prp8p 在两个不同的阶段与 3 剪接位点相互作用。
DOI：
--
发表时间：
1995
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Umen,JG;Guthrie,C
通讯作者：
Guthrie,C
α-裂解蛋白酶突变体广泛特异性的结构基础。
影响因子：
2.9
作者：
Bone,R;Fujishige,A;Kettner,CA;Agard,DA
通讯作者：
Agard,DA
影响因子：
3.3
作者：
H. Hotz;B. Schwer
通讯作者：
H. Hotz;B. Schwer