Synthesis and Characterization of PEGylated Polyethylenimine-Entrapped Gold Nanoparticles for Blood Pool and Tumor CT Imaging
Synthesis and Characterization of PEGylated Polyethylenimine-Entrapped Gold Nanoparticles for Blood Pool and Tumor CT Imaging
复制标题
用于血池和肿瘤 CT 成像的聚乙二醇化聚乙烯亚胺包载金纳米颗粒的合成和表征
DOI：
10.1021/am505006z
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发表时间：
2014-10-08
影响因子：
9.5
通讯作者：
Shi, Xiangyang
中科院分区：
文献类型：
--
作者：
Zhou, Benqing;Zheng, Linfeng;Shi, Xiangyang
报道了用于血池和肿瘤计算机断层扫描 (CT) 成像的聚乙二醇 (PEG) 改性聚乙烯亚胺 (PEI) 中金纳米颗粒 (AuNP) 的合成和表征。在该方法中，部分聚乙二醇化的PEI被用作AuNP合成的模板，然后乙酰化PEI剩余的表面胺。通过不同的方法对合成的聚乙二醇化 PEI 包埋的 AuNPs (Au PENPs) 进行了表征。我们的结果表明，合成的 Au PENP 可以调整为具有 1.9-4.6 nm 范围内的 Au 核心尺寸，并且在研究的浓度范围内具有水溶性、稳定且无细胞毒性。与临床使用的碘化小分子造影剂（例如欧乃派克）相比，所开发的聚乙二醇化 Au PENP 具有更好的 X 射线衰减特性，并且经药代动力学研究证实具有较长的半衰时间（大鼠中 11.2 小时），因此能够实现高效、增强的血池 CT 成像，成像时间长达 75 分钟。同样，由于增强的渗透性和保留效应，聚乙二醇化 Au PENP 也能够用作肿瘤模型有效 CT 成像的造影剂。凭借组织学研究证明的器官生物相容性，所设计的聚乙二醇化金 PENP 有望用作各种生物系统 CT 成像的造影剂。这项研究的意义在于，可以使用具有成本效益的支化聚合物（例如 PEI）作为模板来生成用于 CT 成像应用的功能化 AuNP，而不是使用树枝状聚合物作为模板。
The synthesis and characterization of gold nanoparticles (AuNPs) entrapped within polyethylene glycol (PEG)-modified polyethylenimine (PEI) for blood pool and tumor computed tomography (CT) imaging are reported. In this approach, partially PEGylated PEI was used as a template for AuNP synthesis, followed by acetylating the PEI remaining surface amines. The synthesized PEGylated PEI-entrapped AuNPs (Au PENPs) were characterized via different methods. Our results reveal that the synthesized Au PENPs can be tuned to have an Au core size in a range of 1.9-4.6 nm and to be water-soluble, stable, and noncytotoxic in a studied concentration range. With a demonstrated better X-ray attenuation property than that of clinically used iodinated small molecular contrast agent (e.g., Omnipaque) and the prolonged half-decay time (11.2 h in rat) confirmed by pharmacokinetics studies, the developed PEGylated Au PENPs enabled efficient and enhanced blood pool CT imaging with imaging time up to 75 min. Likewise, thanks to the enhanced permeability and retention effect, the PEGylated Au PENPs were also able to be used as a contrast agent for effective CT imaging of a tumor model. With the proven organ biocompatibility by histological studies, the designed PEGylated Au PENPs may hold great promise to be used as contrast agents for CT imaging of a variety of biological systems. The significance of this study is that rather than the use of dendrimers as templates, cost-effective branched polymers (e.g., PEI) can be used as templates to generate functionalized AuNPs for CT imaging applications.