A genetic linkage map for the tiger pufferfish, Takifugu rubripes
A genetic linkage map for the tiger pufferfish, Takifugu rubripes
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DOI：
10.1534/genetics.105.042051
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发表时间：
2005-09-01
期刊：
影响因子：
3.3
通讯作者：
Suzuki, Y
中科院分区：
文献类型：
--
作者：
Kai, W;Kikuchi, K;Suzuki, Y
虎河豚 Takifugu rubrizes（河豚）的紧凑基因组已测序至“草稿”水平并进行注释以识别所有基因。然而，由于缺乏遗传或物理图谱，基因组序列草案的组装高度碎片化。为了确定序列的长程连锁关系，我们构建了第一个河豚遗传连锁图谱。雄性和雌性的图谱分别跨度为 697.1 和 1213.5 cM，通过亲本中的杂合标记将其排列成 22 个连锁群。生成的图谱由 200 个与基因组序列物理连接的微卫星位点组成，总长度约为 39 Mb。河豚、其他硬骨鱼和哺乳动物的基因组图谱比较表明，硬骨鱼谱系中的同线关系比哺乳动物谱系中更保守。图谱比较还显示河豚谱系特异性的基因组重排导致两个 Hox 基因簇共定位于一个连锁群中。该图谱为开发完整的物理图谱提供了基础，为基因与其他脊椎动物的长程连锁比较提供了基础，并为定位导致泷东方鲀物种之间表型差异的基因座提供了资源。
The compact genome of the tiger pufferfish, Takifugu rubrifies (fugu), has been sequenced to the "draft" level and annotated to identify all the genes. However, the assembly of the draft genome sequence is highly fragmented due to the lack of a genetic or a physical map. To determine the long-range linkage relationship of the sequences, we have constructed the first genetic linkage map for fugu. The maps for the male and female spanning 697.1 and 1213.5 cM, respectively, were arranged into 22 linkage groups by markers heterozygous in both parents. The resulting map consists of 200 microsatellite loci physically linked to genome sequences spanning similar to 39 Mb in total. Comparisons of the genome maps of fugu, other teleosts, and mammals suggest that syntenic relationship is more conserved in the teleost lineage than in the mammalian lineage. Map comparisons also show a pufferfish lineage-specific rearrangement of the genome resulting in colocalization of two Hox gene clusters in one linkage group. This map provides a foundation for development of a complete physical map, a basis for comparison of long-range linkage of genes with other vertebrates, and a resource for mapping loci responsible for phenotypic differences among Takifugu species.