Similarities in Leptospira Serogroup and Species Distribution in Animals and Humans in the Indian Ocean Island of Mayotte
Similarities in Leptospira Serogroup and Species Distribution in Animals and Humans in the Indian Ocean Island of Mayotte
复制标题
DOI：
10.4269/ajtmh.2012.12-0102
复制
发表时间：
2012-07-01
影响因子：
3.3
通讯作者：
Bourhy, Pascale
中科院分区：
文献类型：
--
作者：
Desvars, Amelie;Naze, Florence;Bourhy, Pascale
我们的目的是确定当地的钩端螺旋体病的动物水库，解释钩端螺旋体菌株最近描述的马约特岛的病人之间的不寻常的功能。通过使用当地临床分离株的显微镜凝集试验，我们发现，11.2%的黑鼠钩端螺旋体血清阳性，而10.2%的狐蝠，2%的狐猴，93.1%的家犬，87.5%的流浪狗血清阳性。如在人类中观察到的，Mini是动物中循环的主要血清群，而黄疸出血型血清群不存在。使用定量聚合酶链反应，我们还表明，29.8%的大鼠携带钩端螺旋体在他们的肾脏。通过对黑鼠肾脏中发现的钩端螺旋体16S rRNA基因序列进行测序，鉴定出四种基因型（伯格彼得逊钩端螺旋体、问号钩端螺旋体、克氏钩端螺旋体和钩端螺旋体）。borgpetersenii组B），该研究确定黑鼠是钩端螺旋体病传播给人类的主要来源。这种钩端螺旋体菌株的遗传多样性的起源进行了讨论。
Our objective was to identify local animal reservoirs of leptospirosis to explain the unusual features of Leptospira strains recently described among patients on the island of Mayotte. By means of a microscopic agglutination test using local clinical isolates, we found that 11.2% of black rats were seropositive to Leptospira, whereas 10.2% of flying foxes, 2% of lemurs, 93.1% of domestic dogs, and 87.5% of stray dogs were seropositive. As observed in humans, Mini was the main serogroup circulating in animals, whereas serogroup Icterohaemorrhagiae was absent. Using quantitative polymerase chain reaction, we also showed that 29.8% of rats carried leptospires in their kidneys. The sequencing of 16S rRNA gene sequences of Leptospira found in black rat kidneys identified four genomospecies (Leptospira borgpetersenii, Leptospira interrogans, Leptospira kirschneri, and L. borgpetersenii group B), which established black rats as the major source of leptospirosis transmission to humans. The origins of such a genetic diversity in Leptospira strains are discussed.