Isolation of twelve microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f.sp tritici
Isolation of twelve microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f.sp tritici
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DOI：
10.1046/j.1471-8286.2002.00322.x
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发表时间：
2002-12-01
期刊：
影响因子：
--
通讯作者：
Solignac, M
中科院分区：
文献类型：
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作者：
Enjalbert, J;Duan, X;Solignac, M
我们报道了小麦条锈菌的12个微卫星标记的特征。富集协议被用来分离微卫星位点，多态性进行了探讨，与96株从法国和中国的几个地点收集的自然人群。8个引物对8个小麦疫霉菌株进行扩增，有8个引物（67%）出现了交叉扩增。
We report the characterization of 12 microsatellite markers in the biotrophic fungus Puccinia striiformis f.sp. tritici, responsible for yellow rust on wheat. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with 96 isolates from natural populations collected from several French and Chinese locations. Eight primers (67%) showed cross-amplification when tested with eight isolates of P. triticina.