Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid.
Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid.
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根癌农杆菌肿瘤诱导质粒 T 区发生多处突变。
DOI：
10.1073/pnas.80.6.1660
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发表时间：
1983
影响因子：
11.1
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Ream,LW;Gordon,MP;Nester,EW
影响肿瘤形态的三个遗传基因座位于pTiA 6 NC T-DNA中：tms、tmr和tml。使用缺失和多个转座子插入，我们构建了肿瘤诱导（Ti）质粒，代表每一种可能的双重和三重突变体组合。TMSTMR和TMSTMRTML突变体在大多数植物上不引起肿瘤，并且在少数其它宿主上产生非常弱的应答，但是TMSTMML和TMRTML突变体是毒性的。因此，单独的tms+或tmr+都可以促进显著的肿瘤生长，但tml+本身是不够的。在tms突变体诱导肿瘤并伴随芽增殖的宿主上，添加tml突变体减少或消除芽增殖，表明tml+促进芽发育。由tms tmr和tms tmr tml突变体引发的小愈伤组织含有农杆平，这表明这些植物细胞在没有实质性肿瘤生长的情况下掺入了T-DNA。
Three genetic loci affecting tumor morphology lie within pTiA6NC T-DNA: tms, tmr, and tml. Using deletions and multiple transposon insertions, we constructed tumor-inducing (Ti) plasmids representing every possible double and triple mutant combination. tms tmr and tms tmr tml mutants did not incite tumors on most plants and produced a very weak response on a few other hosts but tms tml and tmr tml mutants were virulent. Thus, either tms+ or tmr+ alone can promote significant tumor growth but tml+ by itself is not sufficient. On hosts where tms mutants induce tumors accompanied by shoot proliferation, addition of a tml mutation reduces or eliminates shoot proliferation, suggesting that tml+ promotes shoot development. The small calli incited by tms tmr and tms tmr tml mutants contain agropine, an indication that these plant cells incorporate T-DNA in the absence of substantial tumor growth.