Correlation of in vitro cytokine responses with the chemical composition of soil-derived particulate matter.
Correlation of in vitro cytokine responses with the chemical composition of soil-derived particulate matter.
复制标题
DOI：
10.1289/ehp.8360
复制
发表时间：
2006-03
影响因子：
10.4
通讯作者：
Yost, Garold S
中科院分区：
文献类型：
--
作者：
Veranth, John M;Moss, Tyler A;Chow, Judith C;Labban, Raed;Nichols, William K;Walton, John C;Watson, John G;Yost, Garold S
我们用10-80 μg/cm 2的灰尘处理BEAS-2B型人肺上皮细胞，这些灰尘来自美国西部的土壤和路面，含有空气动力学直径< 2.5 μm的颗粒物（PM）。细胞活力和细胞因子分泌反应在24 hr. Each粉尘样品是一个复杂的混合物，含有来自不同的矿物颗粒与生物和人为的材料混合。我们使用基于美国环境保护署物种形成趋势网络（U.S. Environmental Protection Agency Speciation Trends Network，简称EPA）和国家公园服务机构间受保护视觉环境监测（Interagency Monitoring of Protected Visual Environments，简称IMPROVE）网络的方法确定了颗粒的化学成分。环境监测网络报告的功能定义的碳分数尚未广泛用于毒理学研究。来自不同地点的土壤来源的PM2.5显示出广泛的体外诱导促炎细胞因子白细胞介素-6（IL-6）和IL-8释放的效力。使用单变量回归和多变量冗余分析来测试活力和细胞因子释放与40种元素、7种离子和8种碳组分的浓度的相关性。颗粒表现出的IL-6释放和元素和可热解碳馏分之间的正相关性，和最强的相关性，涉及地壳元素之间的IL-6释放和铝：硅比。所观察到的土壤和道路来源的粉尘的低挥发性有机成分和BEAS-2B细胞释放的细胞因子之间的相关性是相关的调查机制连接特定的空气污染颗粒类型与引发事件，导致气道炎症敏感人群。
We treated human lung epithelial cells, type BEAS-2B, with 10–80 μg/cm2 of dust from soils and road surfaces in the western United States that contained particulate matter (PM) < 2.5 μm aerodynamic diameter. Cell viability and cytokine secretion responses were measured at 24 hr. Each dust sample is a complex mixture containing particles from different minerals mixed with biogenic and anthropogenic materials. We determined the particle chemical composition using methods based on the U.S. Environmental Protection Agency Speciation Trends Network (STN) and the National Park Service Interagency Monitoring of Protected Visual Environments (IMPROVE) network. The functionally defined carbon fractions reported by the ambient monitoring networks have not been widely used for toxicology studies. The soil-derived PM2.5 from different sites showed a wide range of potency for inducing the release of the proinflammatory cytokines interleukin-6 (IL-6) and IL-8 in vitro. Univariate regression and multivariate redundancy analysis were used to test for correlation of viability and cytokine release with the concentrations of 40 elements, 7 ions, and 8 carbon fractions. The particles showed positive correlation between IL-6 release and the elemental and pyrolyzable carbon fractions, and the strongest correlation involving crustal elements was between IL-6 release and the aluminum:silicon ratio. The observed correlations between low-volatility organic components of soil- and road-derived dusts and the cytokine release by BEAS-2B cells are relevant for investigation of mechanisms linking specific air pollution particle types with the initiating events leading to airway inflammation in sensitive populations.