Species-specific patterns in spatio-temporal dynamics of juvenile chum salmon and their zooplankton prey in Otsuchi Bay, Japan, revealed by simultaneous eDNA quantification of diverse taxa from the same water samples.
Species-specific patterns in spatio-temporal dynamics of juvenile chum salmon and their zooplankton prey in Otsuchi Bay, Japan, revealed by simultaneous eDNA quantification of diverse taxa from the same water samples.
复制标题
通过对同一水样中不同类群的同时 eDNA 定量，揭示了日本大槌湾幼年鲑鱼及其浮游动物猎物的物种特异性时空动态模式。
DOI：
10.1111/fog.12631
复制
发表时间：
2023
影响因子：
2.6
通讯作者：
Susumu Hyodo
中科院分区：
文献类型：
--
作者：
Yuki Minegishi;Marty Kwok-Shing Wong;Mako Nakao;Yuichiro Nishibe;Aiko Tachibana;Yoo-Jun Kim;Susumu Hyodo
为了了解幼年鲑鱼 Oncorhynchus keta 及其浮游动物猎物、浅海拟卡拉努斯纽曼尼 (Pseudocalanus newmani)、海洋尤卡拉努斯邦吉 (Eucalanus bungii) 和 Themisto japonica 的时空动态，我们开发了物种特异性环境 DNA (eDNA) 定量方法。首先，我们使用所开发的检测方法通过胃内容物分析证实了在大槌湾收集的幼年鲑鱼捕食目标浮游动物。对海湾收集的水进行尺寸分数分析表明 P. 纽曼和E. bungiiDNA 主要源自无节幼体和早期桡足类。然后，我们对 2018 年和 2019 年 1 月至 6 月采集的相同水样进行 eDNA 分析，调查了大槌湾浮游动物和幼年鲑鱼的分布和丰度。1 月底至 6 月中旬在海湾中发现了鲑鱼 DNA。 纽曼尼DNA在整个季节都出现在整个海湾，并且在二月至五月期间最为丰富，而其他两个物种的DNA仅在三月至五月期间出现在斑点中，数量较少。浮游动物DNA数量增加的时间与亲潮水入侵海湾的时间非常吻合。此外，环境数据表明P. 纽曼尼鱼也可能在 2019 年通过沿海 Oyashio 水体传播。本研究成功揭示了浮游动物和鲑鱼动态的物种特异性模式，反映了它们的生态特征和海洋条件，并显示了对同一水样中的不同类群进行同步 eDNA 定量的有效性。
To understand the spatio‐temporal dynamics of juvenile chum salmonOncorhynchus ketaand their zooplankton prey, the neriticPseudocalanus newmani, the oceanicEucalanus bungii, andThemisto japonica, we developed species‐specific environmental DNA (eDNA) quantification methods. First, we confirmed that juvenile chum salmon collected in Otsuchi Bay preyed on the target zooplankton by the stomach contents analyses using the developed assays. Size‐fraction analyses of the water collected in the bay showed thatP. newmaniandE. bungiiDNA were derived mostly from nauplii and early copepodids. We then investigated the distribution and abundance of zooplankton and juvenile chum salmon in Otsuchi Bay using eDNA analyses of the same water samples collected from January to June in 2018 and 2019. Chum salmon DNA was found in the bay from the end of January to mid‐June.P. newmaniDNA appeared across the bay throughout the season and were most abundant from February to May, while the other two species DNA were only found in spots, in lower quantities, from March to May. The timings that the zooplankton DNA quantities became greater were corresponded well to that of the Oyashio water intrusion into the bay. In addition, the environmental data indicates thatP. newmaniwas possibly transported also by the Coastal Oyashio water in 2019. The present study successfully revealed species‐specific patterns in the dynamics of zooplankton and chum salmon, reflecting their ecological characteristics and oceanographic conditions, and showed the effectiveness of simultaneous eDNA quantifications for diverse taxa from the same water samples.