Simple, Accurate, Serotype-Specific PCR Assay To Differentiate Streptococcus pneumoniae Serotypes 6A, 6B, and 6C
Simple, Accurate, Serotype-Specific PCR Assay To Differentiate Streptococcus pneumoniae Serotypes 6A, 6B, and 6C
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DOI：
10.1128/jcm.00484-09
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发表时间：
2009-08-01
影响因子：
9.4
通讯作者：
Gilbert, Gwendolyn L.
中科院分区：
文献类型：
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作者：
Jin, Ping;Xiao, Meng;Gilbert, Gwendolyn L.
在这项研究中，我们建立了一种简单、可靠、血清型特异性的PCR方法来区分肺炎链球菌6A、6B和6C型。它比目前用于鉴定6A、6B和6C血清型的方法更有效和实用。从侵袭性肺炎球菌病(n=101)和非侵袭性肺炎球菌病(n=19)患者中选择的120株6号血清群分离株，其中45株经Quellung反应鉴定为6A，75株经Quellung反应鉴定为6B。PCR分析证实了6B血清型分离株的结果，并鉴定了两个不同的亚型。已鉴定为6A型的45株分离物中，有14株实际上属于6C型。
In this study, we developed a simple, reliable, serotype-specific PCR method to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. It was more efficient and practical than the assays currently being used to identify serotypes 6A, 6B, and 6C. Of 120 selected serogroup 6 isolates from subjects with invasive (n = 101) and noninvasive (n = 19) pneumococcal disease, most of which were collected after 2003 in New South Wales, 45 had been identified as 6A and 75 had been identified as 6B by the Quellung reaction. PCR analysis confirmed the results for serotype 6B isolates and identified two different subtypes. Fourteen of 45 isolates that had been identified as serotype 6A actually belonged to serotype 6C.