Increasing substance P levels in serum and synovial tissues from patients with developmental dysplasia of the hip (DDH).
Increasing substance P levels in serum and synovial tissues from patients with developmental dysplasia of the hip (DDH).
复制标题
发育性髋关节发育不良 (DDH) 患者血清和滑膜组织中 P 物质水平升高。
DOI：
10.1186/1471-2474-15-92
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发表时间：
2014-03-19
影响因子：
2.3
通讯作者：
Chen XD
中科院分区：
文献类型：
--
作者：
Wang H;Zheng XF;Zhang X;Li Z;Shen C;Zhu JF;Cui YM;Chen XD
研究背景已证实速激肽能神经递质参与了一系列疾病的炎症过程和慢性疼痛。本研究检测了不同时期DDH患者血清和髋关节滑膜组织中P物质（SP）及其受体NK-1受体（NK-1 R）的水平，方法采用免疫组织化学方法检测DDH及DDH合并骨关节炎（DDH& OA）患者血清及滑膜组织中SP含量，并与疼痛感觉及髋关节功能障碍进行相关性分析。OA）组分别采用免疫组化法、酶联免疫吸附试验（ELISA）和二步醋酸抽提法进行比较。采用免疫组化、实时荧光定量PCR（QRT-PCR）和Western-Blot方法比较滑膜组织中NK-1 R的表达。采用视觉模拟评分（VAS）和Harris髋关节评分（HHS）评定疼痛程度和髋关节功能活动度。结果DDH组、DDH + OA组血清SP水平明显高于正常对照组（P < 0. 05），DDH + OA组血清SP水平明显高于DDH + OA组（P < 0. 05），DDH + OA组血清SP水平明显高于DDH + OA组（P < 0. 05）。免疫组化、QRT-PCR及组织ELISA检测结果显示，DDH + OA组滑膜组织中SP含量高于DDH组。各组血清SP水平无性别差异。滑膜组织中SP含量的增加主要来源于周围神经末梢的分离。DDH和OA患者（男+女）血清SP与VAS、HHS相关。DDH患者（男性）血清SP与HHS相关。DDH和OA患者（男+女）血清SP水平与血沉（ESR）也有相关性。通过免疫印迹、免疫组化和定量RT-PCR.ConclusionsThese研究结果表明，从DDH患者到DDH & OA患者，血清和滑膜组织中SP水平升高，可能与髋关节功能丧失和慢性疼痛感有关。SP沿着其受体NK-1 R可能参与DDH向DDH&OA的发展。在未来，SP和NK-1 R的抑制剂可能代表一种新的药物治疗靶点，用于DDH患者的疼痛缓解，炎症缓解和关节退变延迟。
BackgroundThe tachykininergic neurotransmitters have been proved to be involved in the inflammatory progress and chronic pain in series of disease. The present study was undertaken to evaluate the levels of substance P (SP) and its receptors NK-1 receptor (NK-1R) in both serum and synovial tissues of hip joint from patients with different stages of DDH, and to detect the possible correlation of serum SP levels with pain sensation and dysfunction of the hip joint.MethodsSP levels in serum and synovial tissues from patients with DDH and DDH combined with osteoarthritis (DDH&OA) group were compared through immunohistochemistry (IHC), ELISA, and 2-step acetic acid extraction method respectively. Expression of NK-1R in synovial tissues was compared through IHC, quantitive Real-Time PCR (QRT-PCR) and Western-Blot. The severities of pain sensation and the functional activities of hip joint were assessed by Visual analogue scale (VAS) and Harris hip score (HHS). Correlations of serum SP levels with VAS, HHS and erythrocyte sedimentation rate (ESR) were evaluated respectively in these groups.ResultsSignificantly elevated serum SP levels were detected in group of DDH and DDH&OA compared to that in normal group. IHC, QRT-PCR as well as tissue Elisa showed that SP levels in synovial tissue of DDH&OA group is stronger than that in DDH group. Serum SP levels in each group have no gender differences. The enhanced SP levels in synovial tissue mainly came from the segregation of peripheral nerve endings. Serum SP correlated with VAS and HHS in patients with DDH&OA (Male + Female). Serum SP correlated with HHS in patients with DDH (Male). Serum SP levels also correlated with erythrocyte sedimentation rate (ESR) in patients with DDH&OA (Male + Female). Up-regulated expression of NK-1R was also observed in synovial tissue of patients with DDH&OA compared to patients with DDH, through western-blot, IHC, and QRT-PCR.ConclusionsThese findings indicated that the increasing SP levels in serum and synovial tissues, observed from patients with DDH to patients with DDH&OA, might associate with the loss of function and chronic pain sensation in hip joint. SP along with its receptors NK-1R might be involved in the progression of DDH into DDH&OA. In the future, inhibitors of SP as well as NK-1R may represent a novel pharmacotherapy target for pain relieving, inflammation alleviating and joint degeneration delaying for patients with DDH.
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影响因子：
3.7
作者：
Mitchell AJ;Lone AM;Tinoco AD;Saghatelian A
通讯作者：
Saghatelian A
影响因子：
2.8
作者：
Li, Jian;Kreicbergs, Andris;Ahmed, Mahmood
通讯作者：
Ahmed, Mahmood
影响因子：
2.2
作者：
Nakamura, Junichi;Oinuma, Kazuhiro;Kishida, Shunji
通讯作者：
Kishida, Shunji
影响因子：
4.2
作者：
Albers, Christoph E.;Steppacher, Simon D.;Siebenrock, Klaus A.
通讯作者：
Siebenrock, Klaus A.
DOI：
10.1007/s11420-012-9286-8
发表时间：
2012-10-01
期刊：
HSS journal : the musculoskeletal journal of Hospital for Special Surgery
影响因子：
--
作者：
Al-Moussa, Sulaiman;Moroz, Paul;Beaule, Paul E
通讯作者：
Beaule, Paul E