Involvement of RTE1 in conformational changes promoting ETR1 ethylene receptor signaling in Arabidopsis.
Involvement of RTE1 in conformational changes promoting ETR1 ethylene receptor signaling in Arabidopsis.
复制标题
RTE1参与拟南芥中ETR1乙烯受体信号传导的构象变化。
DOI：
10.1111/j.1365-313x.2008.03615.x
复制
发表时间：
2008-11
期刊：
影响因子：
7.2
通讯作者：
Chang, Caren
中科院分区：
文献类型：
--
作者：
Resnick, Josephine S.;Rivarola, Maximo;Chang, Caren
乙烯是植物生长发育和对环境胁迫反应的重要调节因子。拟南芥感知乙烯通过五个同源受体，负调控乙烯反应。RTE 1是一个在植物、动物和一些原生生物中保守的新基因，最近被鉴定为ETR 1乙烯受体的正调控因子。在这里，我们从遗传学角度分析ETR 1对RTE 1的依赖性，以进一步了解RTE 1的功能。RTE 1的功能被认为是独立的，从RAN 1，编码乙烯受体功能所需的铜转运蛋白不同。我们测试了rte 1功能丧失突变抑制11 etr 1乙烯结合结构域错义突变的能力，所有这些突变都导致了由于组成性信号传导导致的显性乙烯不敏感性。这种抑制测试发现了两类etr 1突变-RTE 1依赖性和RTE 1独立性。这些突变的性质表明，乙烯结合结构域是一个可能的目标RTE 1行动。基于这些发现，我们提出RTE 1通过对乙烯结合结构域的构象效应促进ETR 1信号传导。
Ethylene is an important regulator of plant growth, development and responses to environmental stresses. Arabidopsis perceives ethylene through five homologous receptors that negatively regulate ethylene responses. RTE1, a novel gene conserved in plants, animals and some protists, was recently identified as a positive regulator of the ETR1 ethylene receptor. Here, we genetically analyze the dependence of ETR1 on RTE1 in order to obtain further insight into RTE1 function. The function of RTE1 was found to be independent and distinct from that of RAN1, which encodes a copper transporter required for ethylene receptor function. We tested the ability of an rte1 loss-of-function mutation to suppress 11 etr1 ethylene-binding domain mis-sense mutations, all of which result in dominant ethylene insensitivity due to constitutive signaling. This suppression test uncovered two classes of etr1 mutations – RTE1-dependent and RTE1-independent. The nature of these mutations suggests that the ethylene-binding domain is a possible target of RTE1 action. Based on these findings, we propose that RTE1 promotes ETR1 signaling through a conformational effect on the ethylene-binding domain.