SURFACE-EXPOSED PROTEIN ANTIGENS OF THE GONOCOCCAL OUTER-MEMBRANE
SURFACE-EXPOSED PROTEIN ANTIGENS OF THE GONOCOCCAL OUTER-MEMBRANE
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DOI：
10.1128/iai.34.3.804-816.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
SWANSON, J
中科院分区：
文献类型：
--
作者：
SWANSON, J
将完整的125 I标记淋球菌（GC）与抗完整GC的兔血清一起孵育。洗涤后，将[125 I]GC溶解在去污剂中;通过蛋白A-Sepharose免疫沉淀放射性碘标记的抗原-抗体复合物。可以在用这些抗血清获得的免疫沉淀物中鉴定出几种GC外膜蛋白，包括蛋白质I、II和III。在许多免疫沉淀物中，存在44 K蛋白。抗血清对蛋白II的反应性可以证明，一些兔血清含有非常突出的表观抗体活性对这种蛋白质。蛋白质I和III被发现在免疫沉淀物中的比例相似，这表明它们在GC外膜中形成杂聚物。从用全GC免疫的兔连续获得的血清可以证明抗体反应性和特异性的定性和定量差异。使用活的或福尔马林固定的热灭活葡萄球菌产生125 I标记的GC成分的非免疫沉淀;无论葡萄球菌是否含有蛋白A，都会发生这种情况。
Whole, 125I-labeled gonococci (GC) were incubated with rabbit sera against whole GC. After washing, the [125I]GC were lysed in detergent; radioiodinated antigen-antibody complexes were immunoprecipitated by protein A-Sepharose. Several GC outer membrane proteins, including proteins I, II and III, could be identified in immunoprecipitates obtained with these antisera. In many immunoprecipitates, a 44K protein was present. Reactivity of antisera toward protein II could be demonstrated and some rabbit sera contained very prominent apparent antibody activity toward this protein. Proteins I and III were found in similar ratios in immunoprecipitates, suggesting that they form heteropolymers in GC outer membranes. Qualitative and quantitative differences in antibody reactivity and specificity could be demonstrated with serially obtained sera from rabbits immunized with whole GC. This use of viable or Formalin-fixed heat-killed staphylococci yielded non-immunological precipitation of 125I-labeled GC constituents; this occurred with staphylococci regardless of whether they contained protein A.