Isolation and properties of porcine thyrotropin-releasing hormone.
Isolation and properties of porcine thyrotropin-releasing hormone.
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猪促甲状腺素释放激素的分离和性质。
DOI：
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发表时间：
1969
影响因子：
4.8
通讯作者：
J. F. Barrett
中科院分区：
文献类型：
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作者：
A. Schally;T. Redding;C. Bowers;J. F. Barrett
摘要 描述了从猪下丘脑中制备级分离促甲状腺素释放激素的方法。两批 100,000 和 165,000 下丘脑的产量分别为 2.8 mg 和 4.4 mg。这种具有促甲状腺激素释放激素活性的物质，经计算纯化约570,000倍，在小鼠体内剂量为1 ng时具有活性，在体外剂量为10 pg时刺激大鼠垂体分泌促甲状腺激素。薄层色谱、电泳和纸色谱表明该材料是均质的。组氨酸、谷氨酸和脯氨酸以等摩尔比存在，约占活性物质干重的32％。然而，含有组氨酸、脯氨酸和谷氨酸或谷氨酰胺的 8 种合成肽在 10 µg 的大剂量下没有促甲状腺素释放激素活性。促甲状腺激素释放激素的生物活性被重氮化磺胺酸、N-溴代琥珀酰亚胺或酸水解消除，但不受高碘酸盐或与蛋白水解酶一起孵育的影响。
Abstract Isolation on a preparative scale of thyrotropin-releasing hormone from porcine hypothalami is described. The yields from two batches of 100,000 and 165,000 hypothalami were 2.8 mg and 4.4 mg, respectively. The material with thyrotropin-releasing hormone activity, calculated to be purified about 570,000 times, was active in mice at doses of 1 ng in vivo, and stimulated the secretion of thyroid-stimulating hormone from rat pituitaries in vitro at doses of 10 pg. Thin layer chromatography, electrophoresis, and paper chromatography indicated that the material was homogeneous. Histidine, glutamic acid, and proline, which were present in an equimolar ratio, accounted for about 32% of the dry weight of the active material. However, eight synthetic peptides containing histidine, proline, and glutamic acid or glutamine had no thyrotropin-releasing hormone activity at doses as large at 10 µg. Biological activity of thyrotropin-releasing hormone was abolished by diazotized sulfanilic acid, N-bromosuccinimide, or acid hydrolysis, but was not affected by periodate or by incubation with proteolytic enzymes.