Optimizing a multicolor immunophenotyping assay
Optimizing a multicolor immunophenotyping assay
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DOI：
10.1016/j.cii.2007.05.002
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发表时间：
2007-09-01
影响因子：
1.7
通讯作者：
Roederer, Mario
中科院分区：
文献类型：
--
作者：
Mahnke, Yolanda D.;Roederer, Mario
多色实验可能难以实施和优化;然而，重要的是要花尽可能多的时间尝试变化并详细了解每种试剂（和每个标记步骤）对最终实验中荧光分布的影响。由此产生的数据集将更加可解释，并提供有关实验条件的丰富信息来源。虽然面板的开发仍然主要是经验，我们希望启发式的方法和经验很快将转化为算法，可用于协助开发这样的面板。
Multicolor experiments can be frustratingly difficult to implement and optimize; however, it is important to spend as much time as possible trying out variations and understanding in detail the impact of every reagent (and every labeling step) on the distribution of fluorescences in the final experiment. The resulting data sets will be far more interpretable and provide a rich source of information regarding the experimental conditions. Although panel development still largely is empiric, we hope that heuristic approaches and experience soon will translate into algorithms that can be used to assist in the development of such panels.