Immunopeptides in the defense reactions of Glossina morsitans to bacterial and Trypanosoma brucei brucei infections
Immunopeptides in the defense reactions of Glossina morsitans to bacterial and Trypanosoma brucei brucei infections
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DOI：
10.1016/s0965-1748(02)00029-2
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发表时间：
2002-04-01
影响因子：
3.8
通讯作者：
Bulet, P
中科院分区：
文献类型：
--
作者：
Boulanger, N;Brun, R;Bulet, P
一些双翅目昆虫是引起人类主要传染病的寄生虫的媒介。其中，采采蝇，Glossina属，负责传播锥虫，锥虫是非洲昏睡病的病原体。更好地了解昆虫-寄生虫的相互作用将有助于制定新的战略来对抗这种重要的、往往是致命的疾病。抗菌肽是昆虫在细菌、真菌和寄生虫感染时产生的体液免疫应答的一部分。本文通过分析作为体液免疫反应标志物的AMPS的合成，研究了丝光绿蝇对细菌和布氏锥虫的免疫反应。通过反相色谱、质谱分析、Edman降解和体外抗菌试验，对免疫攻击的G.在Morsitans中，我们确定了三种AMPS：抗菌肽、攻击素和防御素。发现这三种AMP在全身性细菌感染以及细菌和寄生虫经口感染后被诱导。(C)2002爱思唯尔科技有限公司版权所有。
Several dipteran insects are vectors of parasites causing major human infectious diseases. Among these, the tsetse fly, Glossina spp., is responsible for the transmission of trypanosomes, the pathogens responsible for sleeping sickness in Africa. A better understanding of insect-parasite interactions will help establish new strategies to fight this important often fatal disease. Antimicrobial peptides (AMPs) are part of the humoral immune response in insects during bacterial, fungal and parasitic infections. Here, we studied the immune response of Glossina morsitans to bacteria and to Trypanosoma brucei brucei by analyzing the synthesis of AMPS as markers of the humoral immune response. By reversed-phase chromatography, mass spectrometry analysis, Edman degradation and in vitro antimicrobial assays of the hemolymph of immune-challenged adults of G. morsitans, we identified three AMPS: a cecropin, an attacin and a defensin. These three AMPs were found to be induced upon systemic bacterial infection and also after per os infections by bacteria and parasites. (C) 2002 Elsevier Science Ltd. All rights reserved.