Evaluation of clastogenic potential of the antimalarial plant Solanum nudum
Evaluation of clastogenic potential of the antimalarial plant Solanum nudum
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DOI：
10.1002/ptr.1534
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发表时间：
2004-10-01
影响因子：
7.2
通讯作者：
Blair, S
中科院分区：
文献类型：
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作者：
Alvarez, G;Pabón, A;Blair, S
从裸茄中分离得到的化合物具有体外抗恶性疟原虫FCB-2株的活性。从正己烷提取物和水提物中分离得到的主要成分薯蓣皂苷元(C27H4003)进行了微核试验，以测定它们的碎裂潜能。分别以16、32和三种浓度(16、32和g/kg体重)灌胃小鼠(最高浓度对应80%LD_(50))，并以可能的最高浓度(11.187 g/kg体重)接种溶于橄榄油的薯蓣皂苷元。给药后，无论是多染红细胞还是常染红细胞均未观察到微核的发生。有趣的是，在水提取物处理的细胞中，年轻/成熟红细胞比率降低了51%。我们的结论是，薯蓣皂苷元和水提物都没有裂解活性，并且水提物在上述浓度下表现出一定的毒性。这些结果意义重大，因为薯蓣皂苷元可能是治疗疟疾的一种新的治疗选择。版权所有(C)2004 John Wiley Sons，Ltd.
Compounds isolated from Solanum nudum have shown in vitro antimalarial activity against the FCB-2 strain of Plasmodium falciparum. Diosgenone (C27H40O3) the main component isolated from the hexane extract and an aqueous extract were evaluated to measure their clastogenic potential using the micronucleus test. Three concentrations (16, 32 and 64 g/kg of weight) of the aqueous extract were administered intraperitoneally into mice, (the highest concentration corresponded to 80% LD50) and diosgenone solubilized in olive oil was inoculated at the highest concentration possible (11.187 g/kg of weight). After administration of the compounds, no induction of micronucleus was observed either in polychromatic or normochromatic erythrocytes. Interestingly, a reduction of 51% in the young/mature erythrocytes ratio was seen in cells treated with aqueous extract. We conclude that neither diosgenone nor the aqueous extract have clastogenic activity, and that the aqueous extract showed some toxicity at the above mentioned concentrations. These results are significant since diosgenone could be a new therapeutic alternative for the treatment of malaria. Copyright (C) 2004 John Wiley Sons, Ltd.