Porous poly(ethylene glycol)-polyurethane hydrogels as potential biomaterials
Porous poly(ethylene glycol)-polyurethane hydrogels as potential biomaterials
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DOI：
10.1002/pi.4802
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发表时间：
2015-03-01
影响因子：
3.2
通讯作者：
Badiger, Manohar V.
中科院分区：
文献类型：
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作者：
Divakaran, Anumon V.;Torris, Arun A. T.;Badiger, Manohar V.
我们报道了使用 PEG-4000 作为软链段和 4,4-亚甲基双（环己基异氰酸酯）作为硬链段的多孔聚（乙二醇）-聚氨酯（PEG-PU）水凝胶的合成。水凝胶的溶胀程度可以通过改变交联剂（即1,2,6-己三醇）的量来控制。使用固态 C-13 NMR 和傅里叶变换红外光谱对水凝胶进行结构表征。广角 X 射线衍射研究表明存在 PEG 结晶域，小角 X 射线散射研究表明存在层状微结构。为了在水凝胶中产生多孔结构，使用了冷冻干燥的低温处理。水凝胶的扫描电子显微镜和三维微型计算机断层扫描成像表明存在互连的孔。使用动态机械分析测量水凝胶和干凝胶的机械强度。观察到的平衡溶胀凝胶和干燥凝胶的动态储能模量 (E) 分别为 0.15 和 4.2 MPa。有趣的是，多孔 PEG-PU 干凝胶还显示出 5.6 MPa 的 E，表明将孔隙率纳入凝胶基质后具有相似的机械强度。最后，初步的细胞相容性研究表明细胞在水凝胶中增殖的能力。这些凝胶显示出在组织工程中作为支架和植入物的应用前景。 (c) 2014年化学工业学会
We report the synthesis of porous poly(ethylene glycol)-polyurethane (PEG-PU) hydrogels using PEG-4000 as a soft segment and 4,4-methylenebis(cyclohexylisocyanate) as a hard segment. The degree of swelling in the hydrogels could be controlled by varying the amount of crosslinking agent, namely 1,2,6-hexanetriol. Structural characterization of the hydrogels was performed using solid-state C-13 NMR and Fourier transform infrared spectroscopy. Wide-angle X-ray diffraction studies revealed the existence of crystalline domains of PEG and small-angle X-ray scattering studies showed the presence of lamellar microstructures. For generating a porous structure in the hydrogels, cryogenic treatment with lyophilization was used. Scanning electron microscopy and three-dimensional micro-computed tomography imaging of the hydrogels indicated the presence of interconnected pores. The mechanical strength of the hydrogels and xerogels was measured using dynamic mechanical analysis. The observed dynamic storage moduli (E) for the equilibrium swollen and dry gels were found to be 0.15 and 4.2 MPa, respectively. Interestingly, the porous PEG-PU xerogel also showed E of 5.6 MPa indicating a similar mechanical strength upon incorporating porosity into the gel matrix. Finally, preliminary cytocompatibility studies showed the ability of cells to proliferate in the hydrogels. These gels show promise for applications as scaffolds and implants in tissue engineering. (c) 2014 Society of Chemical Industry