Antigenic expression of neuron-associated class III beta-tubulin isotype (h beta 4) and microtubule-associated protein 2 (MAP2) by the human retinoblastoma cell line WERI-Rb1. A comparative immunoblot and immunocytochemical study.
Antigenic expression of neuron-associated class III beta-tubulin isotype (h beta 4) and microtubule-associated protein 2 (MAP2) by the human retinoblastoma cell line WERI-Rb1. A comparative immunoblot and immunocytochemical study.
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人视网膜母细胞瘤细胞系 WERI-Rb1 神经元相关 III 类 β-微管蛋白同种型 (h beta 4) 和微管相关蛋白 2 (MAP2) 的抗原表达。
DOI：
10.1159/000267002
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发表时间：
1990
影响因子：
2.1
通讯作者：
Rubinstein,LJ
中科院分区：
文献类型：
--
作者：
Gass,P;Frankfurter,A;Katsetos,CD;Herman,MM;Donoso,LA;Rubinstein,LJ
在人视网膜母细胞瘤细胞系 WERI-Rbl（在三种不同的体外条件下维持长达 30 天）的比较免疫印迹和免疫细胞化学研究中，评估了两种神经元相关微管蛋白（III 类 β-微管蛋白同种型 (hβ4) 和微管相关蛋白 2 (MAP2)）的抗原表达。对悬浮悬浮液、明胶海绵基质和盖玻片上生长的细胞的完整十二烷基硫酸钠提取物进行蛋白质印迹。对基质培养物进行免疫过氧化物酶组织化学分析。免疫印迹证明 hβ4 和 MAP2 在所有培养条件下均存在。通过免疫细胞化学，在明胶海绵基质外植体上发现细胞学未分化细胞被抗 hβ4 和抗 MAP2 单克隆抗体染色。相反，免疫印迹或免疫细胞化学均未检测到神经胶质原纤维酸性蛋白。这些发现与该细胞系的唯一神经母细胞性质一致，并且没有提供其不同（即神经元和神经胶质）分化能力的证据。
The antigenic expression of two neuron-associated microtubule proteins, class III β-tubulin isotype (hβ4) and microtubule-associated protein 2 (MAP2), was evaluated in a comparative immunoblot and immunocytochemical study of the human retinoblastoma cell line WERI-Rbl maintained for up to 30 days in three different in vitro conditions. Western blots were performed on whole sodium dodecyl sulfate extracts of cells grown in floating suspensions, on Gelfoam matrices and on coverslips. Immunoperoxidase histochemistry was performed on matrix cultures. Immunoblotting demonstrated that hβ4 and MAP2 were present under all culture conditions. By immunocytochemistry, staining of cytologically undifferentiated cells with anti-hβ4 and anti-MAP2 monoclonal antibodies was found on Gelfoam matrix explants. In contrast, glial fibrillary acidic protein was not detected by either immunoblots or immunocytochemistry. These findings are in keeping with the solely neuroblastic nature of this line and provide no evidence for its divergent (i.e. neuronal and glial) differentiation capacity.