Multiple cis-acting sites positively regulate Escherichia coli nrd expression.
Multiple cis-acting sites positively regulate Escherichia coli nrd expression.
复制标题
多个顺式作用位点正向调节大肠杆菌 nrd 表达。
DOI：
10.1046/j.1365-2958.1998.00897.x
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发表时间：
1998
影响因子：
3.6
通讯作者：
Fuchs,JA
中科院分区：
文献类型：
--
作者：
Jacobson,BA;Fuchs,JA
Regulation ofnrdexpression inEscherichia colibycis‐acting elements was found to be more complex than previously reported. At least five upstream sites appear to positively regulatenrdexpression including a Fis binding site, a DnaA binding site, an AT‐rich region, an inverted repeat and a 10 bp site between the AT‐rich region and the inverted repeat. Double mutants defective in these sites indicate that all sites tested act independently when regulatingnrdexpression. As the decrease innrdexpression in exponentially growing cultures paralleled the decrease observed in DNA synthesis‐inhibited cultures for all single and double mutants, we concluded thatnrdis regulated by the same mechanism in these physiological states. As mutants unable to inducenrdexpression during inhibition of DNA synthesis also fail to exhibit cell cycle‐regulatednrdexpression, we conclude that cell cyclenrdregulation is controlled by these same sites. Site‐directed mutagenesis was used to show that the absence of an increase innrdexpression during DNA inhibition previously observed for deletion of the AT‐rich region results from deletion of both the Fis binding site and the AT‐rich region.
