A human RNase E-like activity that cleaves RNA sequences involved in mRNA stability control.
A human RNase E-like activity that cleaves RNA sequences involved in mRNA stability control.
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一种人类 RNase E 样活性，可切割参与 mRNA 稳定性控制的 RNA 序列。
DOI：
10.1073/pnas.92.16.7322
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发表时间：
1995
影响因子：
11.1
通讯作者：
vonGabain,A
中科院分区：
文献类型：
--
作者：
Wennborg,A;Sohlberg,B;Angerer,D;Klein,G;vonGabain,A
我们在人细胞提取物中检测到一种内切核糖核酸酶活性，它能以与来自大肠杆菌的RNase E相同的特异性处理大肠杆菌9 S RNA和外膜蛋白A（ompA）mRNA。杆菌通过离子交换色谱法对人体酶进行了部分纯化，活性部分含有一种蛋白质，该蛋白质可以被识别大肠杆菌的抗体检测到。coli RNase E.含有去稳定基序AUUUA的四个重复序列的RNA和人c-myc mRNA 3'非翻译区的RNA也被E.大肠杆菌RNase E和它的人类对应物的一种方式，可能表明这种活动在哺乳动物mRNA衰变中的作用。还发现含有多于一个AUUUA基序的RNA比仅具有一个或突变基序的RNA更有效地被切割。这一发现的真核细胞的核糖核酸内切活性对应于RNase E表明mRNA降解系统的组件的进化保守。
We have detected an endoribonucleolytic activity in human cell extracts that processes the Escherichia coli 9S RNA and outer membrane protein A (ompA) mRNA with the same specificity as RNase E from E. coli. The human enzyme was partially purified by ion-exchange chromatography, and the active fractions contained a protein that was detected with antibodies shown to recognize E. coli RNase E. RNA containing four repeats of the destabilizing motif AUUUA and RNA from the 3' untranslated region of human c-myc mRNA were also found to be cleaved by E. coli RNase E and its human counterpart in a fashion that may suggest a role of this activity in mammalian mRNA decay. It was also found that RNA containing more than one AUUUA motif was cleaved more efficiently than RNA with only one or a mutated motif. This finding of a eukaryotic endoribonucleolytic activity corresponding to RNase E indicates an evolutionary conservation of the components of mRNA degradation systems.