Micro-/Nano-sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage
Micro-/Nano-sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage
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微/纳米羟基磷灰石引导大鼠骨髓来源的间充质干细胞向成骨细胞谱系分化
DOI：
10.1039/c2nr12072k
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发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Huang, Yan;Zhou, Gang;Fan, Yubo
由细胞和材料组成的再生医学为组织器官的修复和再生提供了新的途径。纳米生物材料因其相对于传统微米材料的优势而备受关注。本研究旨在探讨微米/纳米羟基磷灰石（mu/n-HA）对大鼠骨髓间充质干细胞（rBMSCs）成骨分化的影响。分别采用微波合成法和沉淀法制备了mu/n-HA。采用IR、XRD、SEM、TEM等手段对不同粒径的mu/n-HA进行表征，并与rBMSCs共培养。结果表明，rBMSCs表达更高水平的成骨细胞相关标志物的n-HA比mu-HA刺激。HA的大小是影响rBMSCs成骨分化的重要因素。这为干细胞分化机制的研究提供了新的途径，也为获得更多定向分化的细胞提供了新的途径。
Regenerative medicine consisting of cells and materials provides a new way for the repair and regeneration of tissues and organs. Nano-biomaterials are highlighted due to their advantageous features compared with conventional micro-materials. The aim of this study is to investigate the effects of micro-/nano-sized hydroxyapatite (mu/n-HA) on the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs). mu/n-HA were prepared by a microwave synthesizer and precipitation method, respectively. Different sizes of mu/n-HA were characterized by IR, XRD, SEM, TEM and co-cultured with rBMSCs. It was shown that rBMSCs expressed higher levels of osteoblast-related markers by n-HA than mu-HA stimulation. The size of HA is an important factor for affecting the osteogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.