INFLUENCE OF SEVERAL GROWTH-REGULATORS AND AMINO-ACIDS ON INVITRO ORGANOGENESIS OF TORENIA-FOURNIERI LIND
INFLUENCE OF SEVERAL GROWTH-REGULATORS AND AMINO-ACIDS ON INVITRO ORGANOGENESIS OF TORENIA-FOURNIERI LIND
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DOI：
10.1093/jxb/30.1.27
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发表时间：
1979-01-01
影响因子：
6.9
通讯作者：
HARADA, H
中科院分区：
文献类型：
--
作者：
KAMADA, H;HARADA, H
本文研究了几种生长调节剂和氨基酸对离体培养的红腹锦鸡儿器官发生的影响。佛涅里山使用结间节段测定。用2，4-D处理导致结瘤愈伤组织形成，而NAA [萘乙酸]和IAA不断诱导根，但很少诱导芽。BA [6-苄基腺嘌呤]、玉米素和4-PU [N-苯基-N“-4-吡啶基脲]单独诱导芽的形成，但这些芽不能进一步发育。NAA或2，4-D同时施用对细胞分裂素诱导芽的形成有影响，而IAA不影响，BA与NAA或2，4-D同时施用时，其影响程度降低。当玉米素或激动素与NAA一起添加时，诱导了大量的根。在以KNO_3为主要氮源的确定培养基中，研究了不同L-氨基酸对离体器官发生的影响。在含有NAA和BA的培养基中，丙氨酸和天冬酰胺对芽的形成有显著的促进作用，谷氨酸对芽的形成有一定的促进作用。除谷氨酸和天冬氨酸外，其它氨基酸均抑制根的形成。在含NAA但不含BA的培养基中，脯氨酸、丙氨酸、谷氨酰胺、谷氨酸和天冬氨酸对生根有显著的促进作用，精氨酸和色氨酸对生根有一定的促进作用。谷氨酸和天冬氨酸也促进芽的形成。
The effects of several growth regulators and amino acids in vitro organogenesis of T. fournieri Lind. were determined using internodal segments. Treatment with 2,4-D resulted in nodular callus formation, while NAA [naphthaleneacetic acid] and IAA induced roots constantly but only infrequently induced shoot buds. Individually BA [6-benzyladenine], zeatin and 4-PU [N-phenyl-N''-4-pyridyl-urea] induced bud formation but these shoot buds did not develop further. Formation of buds by cytokinin was influenced by a simultaneous application of NAA or 2,4-D but not of IAA, its degree being reduced when BA was simultaneously applied with NAA or 2,4-D. When zeatin or kinetin was added with NAA, numerous roots were induced. The effects of various L-amino acids on in vitro organogenesis were also investigated using the defined medium in which KNO3 was a principal source of N. The formation of buds was considerably stimulated by alanine and asparagine and slightly by glutamic acid in the medium containing both NAA and BA. All amino acids except for glutamic acid and aspartic acid inhibited root formation in this medium. Root formation was greatly stimulated by proline, alanine, glutamine, glutamic acid and aspartic acid and slightly by arginine and tryptophan in the medium containing NAA but no BA. Glutamic acid and aspartic acid also enhanced bud formation in this medium.