Dual symbiosis in the cold-seep thyasirid clam Maorithyas hadalis from the hadal zone in the Japan Trench, western Pacific
Dual symbiosis in the cold-seep thyasirid clam Maorithyas hadalis from the hadal zone in the Japan Trench, western Pacific
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DOI：
10.3354/meps214151
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发表时间：
2001-01-01
影响因子：
2.5
通讯作者：
Kojima, S
中科院分区：
文献类型：
--
作者：
Fujiwara, Y;Kato, C;Kojima, S
对采自日本海沟深渊区的海蛤Maorithyas hadalis的细菌内共生体进行了表征。通过分子系统发育分析和原位杂交，在鳃组织中发现了两种不同的内共生细菌。共生体I型属于来自深海热液喷口和冷泉的囊蛤和深海贻贝的硫自养共生体。共生体II型与以前报道的细菌共生体无关，与自由生活的化能自养细菌（硫微螺旋菌属和氢弧菌属）有远亲关系。原位杂交实验表明2 M之间的空间分区。hadalis共生体，共生体I主要发生在细菌细胞带的外部区域，共生体II主要位于细菌细胞带的内部区域。这是有史以来最深的化学合成共生记录和第一份报告，以显示海洋无脊椎动物的细胞内共生体之间的空间分区。
The bacterial endosymbionts of the thyasirid clam Maorithyas hadalis collected from the hadal zone in the Japan Trench were characterized. Two distinct phylotypes of endosymbiotic bacteria were discovered within the gill tissues by molecular phylogenetic analysis and in situ hybridization. Symbiont Type I was affiliated with thioautotrophic symbionts of vesicomyid clams and deep-sea mussels from deep-sea hydrothermal vents and cold seeps. Symbiont Type II was not related to previously reported bacterial symbionts, and was distantly related to the free-living chemoautotrophic bacteria (genera Thiomicrospira and Hydrogenovibrio). In situ hybridization experiments indicated spatial partitioning between the 2 M. hadalis symbionts, with Symbiont I occurring mainly in the outer regions of bacteriocyte zones and Symbiont II situated predominantly within inner regions of bacteriocyte zones. This is the deepest chemosynthetic symbiosis ever recorded and the first report to show spatial partitioning between the intracellular endosymbionts in marine invertebrates.