Identification of the immediate-early genes of white spot syndrome virus
Identification of the immediate-early genes of white spot syndrome virus
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白斑综合症病毒立即早期基因的鉴定
DOI：
10.1016/j.virol.2008.12.007
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发表时间：
2009-03-01
期刊：
影响因子：
3.7
通讯作者：
Yan, Xiumin
中科院分区：
文献类型：
--
作者：
Li, Fang;Li, Mingyuan;Yan, Xiumin
在病毒感染过程中，病毒即时早期（IE）基因编码对病毒生命周期至关重要的调节蛋白。本研究利用环己亚胺（CHX）处理的小龙虾血细胞原代培养物和WSSV基因组切片芯片筛选白斑综合征病毒（WSSV） IE基因。通过RT-PCR和时间过程研究鉴定并证实了16个orf，其中包括已知的WSSV IE基因（ie1/wsv069）。鉴定的16种IE蛋白包含4种蛋白(WSV051；WSV069, WSV100, WSV079)具有转录活性，一个（WSV083）具有Ser/Thr激酶结构域，一个（WSV249）先前被描述为泛素E3连接酶。此外，大多数鉴定的WSSV IE基因集中在14 kb的基因组区域（WSSV中国分离物：36,052至50,300 bp）。这种排列方式有利于IE基因的协调控制和快速表达。(c) 2008爱思唯尔公司版权所有。
During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. Here we screened white spot syndrome virus (WSSV) IE genes with cycloheximide (CHX)-treated primary culture of crayfish hemocyte and a WSSV genome tiling microarray. Sixteen ORFs, including a known WSSV IE gene (ie1/wsv069), were identified and confirmed by RT-PCR and time course studies. The 16 identified IE proteins contain four proteins (WSV051. WSV069, WSV100, WSV079) with transcription activity, one (WSV083) with Ser/Thr kinase domain and one (WSV249) previously described to function as an ubiquitin E3 ligase. Furthermore, most of the identified WSSV IE genes cluster in a 14 kb genomic region (WSSV China isolate: 36,052 to 50,300 bp). This type of arrangement may facilitate the coordinate control and rapid expression of IE genes. (c) 2008 Elsevier Inc. All rights reserved.