Spatiotemporal uptake characteristics of [18]F-2-fluoro-2-deoxy-D-glucose in a rat middle cerebral artery occlusion model.
Spatiotemporal uptake characteristics of [18]F-2-fluoro-2-deoxy-D-glucose in a rat middle cerebral artery occlusion model.
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DOI：
10.1161/strokeaha.113.000903
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发表时间：
2013-08
期刊：
影响因子：
8.3
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Yuan H;Frank JE;Hong Y;An H;Eldeniz C;Nie J;Bunevicius A;Shen D;Lin W
脑缺血时脑内葡萄糖代谢的变化是可以预见的。然而，在急性缺血期间，大脑葡萄糖代谢紊乱的详细时空特征在很大程度上仍不清楚。本研究旨在利用正电子发射断层成像技术，特别是在缺血区，描述[18]F-2-氟-2-脱氧-D-葡萄糖(FDG)摄取的时空分布及其与组织预后的关系。采用线栓法制作大鼠局灶性脑缺血模型(n=48)。所有动物均在大脑中动脉闭塞后不同时间(30~150min)行MRI和FDG正电子发射断层扫描。MR和正电子发射断层扫描图像被配准。评估缺血区周围FDG摄取与大脑中动脉闭塞持续时间、脑血流量、表观扩散系数和24小时T2病变的关系。在假定为低FDG摄取率的缺血核心周围的缺血区，持续观察到FDG摄取量升高。高摄取区的空间体积和摄取水平与大脑中动脉闭塞时间呈负相关。高摄取区较对侧脑血流量(28.2±3.2%)和表观弥散系数(9.1±1.4%)轻度降低。共定位分析显示，在再灌注时，高摄取体积的平均12.1±1.7%被招募到最终的脑梗塞中。在急性脑缺血期间，可持续观察到缺血区周围FDG摄取的增加。FDG摄取升高的区域可能反映了可以通过再灌注挽救的活性组织。因此，急性FDG正电子发射断层成像可能在急性卒中患者的治疗中有希望。
Alterations of cerebral glucose metabolism are well anticipated during cerebral ischemia. However, detailed spatiotemporal characteristics of disturbed cerebral glucose metabolism during acute ischemia remain largely elusive. This study aims to delineate spatiotemporal distributions of [18]F-2-fluoro-2-deoxy-D-glucose (FDG) uptake using positron emission tomography imaging, particularly at the peri-ischemic zone, and its correlation with tissue outcome. The intraluminal suture middle cerebral artery occlusion model was used to induce focal cerebral ischemia in rats (n=48). All animals underwent sequential MRI and FDG positron emission tomography imaging at different times (30–150 minutes) after middle cerebral artery occlusion. MR and positron emission tomography images were coregistered. FDG uptake in the peri-ischemic zone was assessed in relation to middle cerebral artery occlusion duration, cerebral blood flow, apparent diffusion coefficient, and 24-hour T2 lesions. Elevated FDG uptake was consistently observed at the peri-ischemic zone surrounding the presumed ischemic core with low FDG uptake. Both the spatial volume and the uptake level of the hyper-uptake region were inversely correlated with the duration of middle cerebral artery occlusion. The hyper-uptake regions exhibited a mild reduction of cerebral blood flow (28.2±3.2%) and apparent diffusion coefficient (9.1±1.4%) when compared with that in the contralateral hemisphere. Colocalization analysis revealed that, with reperfusion, an average of 12.1±1.7% of the hyper-uptake volume was recruited into final infarction. Elevated FDG uptake at the peri-ischemic zone is consistently observed during acute cerebral ischemia. The region with elevated FDG uptake likely reflects viable tissues that can be salvaged with reperfusion. Therefore, acute FDG positron emission tomography imaging might hold promise in the management of patients with acute stroke.