INTERFERON-GAMMA REGULATES AN ANTIGEN SPECIFIC FOR ENDOTHELIAL-CELLS INVOLVED IN LYMPHOCYTE TRAFFIC
INTERFERON-GAMMA REGULATES AN ANTIGEN SPECIFIC FOR ENDOTHELIAL-CELLS INVOLVED IN LYMPHOCYTE TRAFFIC
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DOI：
10.1073/pnas.83.23.9114
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
BUTCHER, EC
中科院分区：
文献类型：
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作者：
DUIJVESTIJN, AM;SCHREIBER, AB;BUTCHER, EC
局部血管分化的一个最显著的例子是由介导循环淋巴细胞从血液中外渗的特化淋巴器官小静脉所展示的。这些血管的特征在于立方形或“高”内皮细胞形态，并且在与迁移的淋巴细胞相互作用的功能能力方面是独特的，调节淋巴细胞通过身体特定区域运输的速率和特异性。我们在这里描述了一种单克隆抗体，MECA-325，它定义了一种内皮细胞分化抗原，选择性地表达在小鼠的高内皮细胞上。此外，我们证明了MECA-325抗原可以在小鼠肺或骨髓衍生的内皮细胞系中由干扰素-γ体外诱导。但不被干扰素-β，白细胞介素-1或内皮细胞有丝分裂原。结果定义了一个独特的标志物与分化的内皮细胞介导的淋巴细胞运输从血液中，他们提供的证据表明，这些高内皮细胞的特化表型可以诱导和控制的局部因素与免疫活性。
One of the most striking examples of localized vascular differentiation is exhibited by specialized lymphoid organ venules that mediate the extravasation of circulating lymphocytes from the blood. These vessels are characterized by cuboidal or "high" endothelial cell morphology and are unique in their functional capacity to interact with migrating lymphocyes, regulating both the rate and specificity of lymphocyte traffic through particular regions of the body. We describe here a monoclonal antibody, MECA-325, that defines an endothelial cell differentiation antigen selectively expressed on high endothelium in the mouse. Thus an antigen defining a specific functional subset of endothelial cells has been found. Furthermore, we demonstrate that the MECA-325 antigen can be induced in mouse lung or bone marrow-derived endothelial cell lines in vitro by interferon-.gamma. but not by interferon-.beta., interleukin-1, or endothelial cell mitogens. The results define a unique marker associated with differentiated endothelial cells mediating lymphocyte traffic from the blood, and they provide evidence that the specialized phenotype of these high endothelial cells may be induced and controlled by local factors associated with immune activity.