Prolonged allergen challenge in murine nasal allergic rhinitis: Nasal airway remodeling and adaptation of nasal airway responsiveness
Prolonged allergen challenge in murine nasal allergic rhinitis: Nasal airway remodeling and adaptation of nasal airway responsiveness
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DOI：
10.1097/mlg.0b013e318033f9b0
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发表时间：
2007-05-01
期刊：
影响因子：
2.6
通讯作者：
Kaga, Kimitaka
中科院分区：
文献类型：
--
作者：
Nakaya, Muneo;Dohi, Makoto;Kaga, Kimitaka
背景：过敏性鼻炎中存在鼻气道重塑，但其范围似乎远不如哮喘广泛。然而，关于鼻气道重塑的研究很少，也没有使用小鼠模型的研究。据报道，在慢性小鼠哮喘模型中，长期过敏原激发后气道高反应性随着重塑的进展而降低。然而，尚未研究鼻过敏中重塑与气道反应性的关系。因此，我们进行了这项研究，以表征长期过敏原挑战后鼻气道结构变化的特征，并检查鼻气道高反应性与重塑之间的关系。方法。我们为卵清蛋白制备了小鼠过敏性鼻炎。随后，从第 19 天到第 53 天、第 88 天和第 130 天，小鼠每周接受卵清蛋白攻击 3 次。我们使用增强暂停系统检查了过敏原引起的鼻部症状和客观鼻部高反应性。此外，还研究了过敏原激发后的病理变化。结果：延长的过敏原激发方案导致显着的鼻气道重塑。具体而言，重塑的特征是杯状细胞增生和粘膜下区域胶原沉积。过敏原引起的鼻部高反应性首先增加，但在长期过敏原挑战后，鼻部症状和鼻部症状逐渐减轻。结论。我们已经证明，小鼠过敏性鼻炎模型中鼻粘膜的重塑延长了过敏原暴露时间。此外，长期暴露于过敏原会导致鼻高反应性减少以及鼻重塑的进展。
Background: Nasal airway remodeling exists in allergic rhinitis, but it appears to be far less extensive than in asthma. However, there has been little study about nasal airway remodeling and no study using mice models. It has been reported that airway hyperresponsiveness decreased after prolonged allergen challenge in a chronic murine asthma model together with the progression of remodeling. However, there has been no study of the relation of remodeling and airway responsiveness in nasal allergy. Therefore, we have undertaken this investigation to characterize nasal airway structural changes after prolonged allergen challenge and to examine the relationship between nasal airway hyperresponsivity and remodeling. Methods. We prepared murine allergic rhinitis for ovalbumin. Mice were subsequently challenged three times a week with ovalbumin from day 19 to days 53, 88, and 130. We examined allergen-induced nasal symptoms and objective nasal hyperresponsiveness using the enhanced pause system. Moreover, the pathologic changes were investigated after allergen challenge. Results: The extended allergen challenge protocol caused significant nasal airway remodeling. Specifically, remodeling was characterized by goblet cell hyperplasia and deposition of collagen in the submucosal area. Allergen-induced nasal hyperresponsiveness was first increased but gradually decreased in nasal symptoms and Penh after prolonged allergen challenge. Conclusions. We have demonstrated that a remodeling of nasal mucosa in a murine allergic rhinitis model prolonged allergen exposure. Moreover, prolonged allergen exposure induced a reduction of nasal hyperresponsiveness together with a progression of nasal remodeling.