An electrochemical biosensor for the detection of epithelial-mesenchymal transition
An electrochemical biosensor for the detection of epithelial-mesenchymal transition
复制标题
用于检测上皮间质转化的电化学生物传感器
DOI：
10.1038/s41467-019-14037-w
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发表时间：
2020-01-10
影响因子：
16.6
通讯作者：
Zhou, Jun
中科院分区：
文献类型：
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作者：
Du, Xin;Zhang, Zhenhua;Zhou, Jun
上皮-间质转化（EMT）是一种重要的生物学过程。电化学传感为开发更有效的EMT检测技术提供了潜力。本研究利用量子点-纳米复合材料的独特性能，建立了一种能够特异性检测E-cadherin变化并分析EMT不同阶段的电化学生物传感器。由于分子信息传输到电子设备，EMT的信号被大大放大。此外，差示脉冲伏安法表明，由于量子点和碳纳米管-金纳米颗粒的协同作用，电化学信号响应迅速灵敏。因此，我们的研究表明，电化学传感是一种有效的技术，用于检测EMT，并可能在分析各种细胞类型的转变有广泛的应用。
Epithelial-mesenchymal transition (EMT) is critically involved in a variety of biological processes. Electrochemical sensing offers potential to develop more effective technology for EMT detection. In this study, by using the unique performance of quantum dot (QD)-nanocomposite materials, we establish an electrochemical biosensor that can specifically detect the change of E-cadherin and analyze different stages of EMT. The signal for EMT is largely magnified due to the transmission of molecular information to the electronic device. In addition, differential pulse voltammetry reveals that the response of the electrochemical signals is rapid and sensitive, due to the synergistic effect of QDs and carbon nanotube-gold nanoparticles. Our study thus suggests that electrochemical sensing is an effective technology for detecting EMT and may have broad applications in analyzing various cell type transitions.