Rapid detection of ketamine and norketamine in rat hair using micropulverized extraction and ultra-performance liquid chromatography-electrospray ionization mass spectrometry.
Rapid detection of ketamine and norketamine in rat hair using micropulverized extraction and ultra-performance liquid chromatography-electrospray ionization mass spectrometry.
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DOI：
10.1002/bmc.1256
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发表时间：
2009-12
期刊：
影响因子：
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通讯作者：
Shinsuke Inagaki;Hatsune Makino;T. Fukushima;J. Min;T. Toyo′oka
中科院分区：
文献类型：
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作者：
Shinsuke Inagaki;Hatsune Makino;T. Fukushima;J. Min;T. Toyo′oka
将微粉萃取与超高效液相色谱-电喷雾电离质谱法相结合，建立了同时快速检测大鼠毛发中氯胺酮及其主要代谢物去甲氯胺酮的新方法。采用反相超高效毛细管电泳法，氯胺酮和去甲氯胺酮在2min内得到很好的分离。以氯胺酮为原料，对微粉提取条件进行了优化，建立的方法检出限为1.7 pg/mg毛发，定量限为5.7 pg/mg毛发。该方法的精密度略好于传统的酸性甲醇萃取法。使用这种方法，洗涤后的老鼠毛的分析可以在16-17分钟内完成。这种方法不仅有望用于分析老鼠的头发样本，也有望用于分析滥用氯胺酮的人的头发样本。
A new method for the rapid and simultaneous detection of ketamine and its major metabolite, norketamine, in rat hair has been developed by combining micropulverized extraction and ultraperformance liquid chromatography-electrospray ionization mass spectrometry. By using reversed-phase UPLC, ketamine and norketamine were well separated within 2 min. Using ketamine-dosed rat hair, the conditions for micropulverized extraction were optimized, and the limits of detection and quantification of the developed method were found to be 1.7 and 5.7 pg/mg hair for ketamine, respectively. The precisions achieved with this method were slightly better than that obtained with conventional acidic methanol extraction method. Using this proposed method, analysis of the washed rat hair could be completed within 16-17 min. This method is expected to be applied for the analysis of the hair samples of not only rats but also ketamine abusers.