Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness
Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness
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DOI：
10.1016/j.biomaterials.2007.06.014
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发表时间：
2007-10-01
期刊：
影响因子：
14
通讯作者：
Reichert, William M.
中科院分区：
文献类型：
--
作者：
Kim, Dong-Hwan;Novak, Matt T.;Reichert, William M.
这项体外研究表征了暴露于可吞噬Ti颗粒（0.78 +/- 0.12 μ m）和表面粗糙度相当的Ti圆盘的人单核细胞的时间细胞因子表达谱。将人THP-1单核细胞培养于六孔组织培养聚苯乙烯（TCPS）板中。每个孔要么是裸露的，要么含有钛颗粒（这些颗粒明显被单核细胞吞噬），要么含有一个钛圆盘。一半的孔用1 μ g/mL脂多糖（LPS）处理，另一半不刺激。裸孔中未刺激和lps刺激的细胞分别为阴性和阳性对照。在1、6、24、48和72 h时从每个孔中取样上清，并使用Luminex系统检测9种不同细胞因子的表达。三种细胞因子（IL-1 β， GM-CSF和IL-13）在所有条件下几乎没有反应，而六种细胞因子（tnf - α, IL-6, MIP-1 α, MCP-1， VEGF和IL-1ra）明显可检测到。表达水平随着培养时间、颗粒浓度和LPS刺激而增加。最重要的是，发现经钛盘处理的细胞在许多情况下比颗粒产生更高的细胞因子表达。(c) 2007 Elsevier Ltd.版权所有。
This in vitro study characterized the temporal cytokine expression profile from human monocytes exposed to phagocytosable Ti particles (0.78 +/- 0.12 mu m) and to Ti discs of comparable surface roughness. Human THP-1 monocytes were cultured in six well tissue culture polystyrene (TCPS) plates. Each well was either bare, contained Ti particles (the particles were clearly engulfed by the monocytes), or contained a Ti disc. Half of the wells were treated with 1 mu g/mL lipopolysaccha ride (LPS), while the other half were left unstimulated. Unstimulated and LPS-stimulated cells in bare wells were the negative and positive controls, respectively. Supernatant was sampled from each well at 1, 6, 24, 48, and 72 h and assayed for the expression of nine different cytokines using a Luminex system. Three cytokines (IL-1 beta, GM-CSF and IL-13) gave little to no response under all conditions, while six cytokines (TNF-alpha, IL-6, MIP-1 alpha, MCP-1, VEGF, and IL-1ra) were clearly detectable. Expression levels generally increased with culture time, particle concentration, and LPS stimulation. Most significantly, it was found that cells treated by Ti discs produced in many instances a higher cytokine expression than did particles. (c) 2007 Elsevier Ltd. All rights reserved.