Cell-cycle-specific inhibition by chloramphenicol of septum fromation and cell division in synchronized cells of Bacillus subtilis
Cell-cycle-specific inhibition by chloramphenicol of septum fromation and cell division in synchronized cells of Bacillus subtilis
复制标题
氯霉素对枯草芽孢杆菌同步化细胞中隔膜形成和细胞分裂的细胞周期特异性抑制
DOI：
10.1128/jb.141.2.502-507.1980
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发表时间：
1980
影响因子：
3.2
通讯作者：
Y. Maruyama
中科院分区：
文献类型：
--
作者：
Y. Miyakawa;T. Komano;Y. Maruyama
利用枯草芽孢杆菌168同步化细胞研究了蛋白质合成与细胞分裂过程的关系。在同步生长开始时加入氯霉素阻止了隔膜的形成和细胞分裂，这表明细胞分裂过程需要蛋白质合成。将药物添加到不同细胞龄的细胞中的实验表明，启动隔膜形成所需的蛋白质合成在约15分钟内完成，细胞分裂所需的蛋白质合成在约45分钟内完成。通过解释蛋白质合成转变点的概念的结果，表明B的细胞分裂过程。枯草芽孢杆菌需要至少两种在细胞周期不同阶段合成的蛋白质分子。这一点得到了实验结果的支持，在该实验中，饥饿和向指数生长的细胞再添加所需的氨基酸诱导了两个同步细胞分裂步骤。此外，这两个转变点与通过抑制异步细胞中蛋白质合成后的剩余分裂获得的估计一致。同时还研究了染色体复制完成的时间与两个转变点的关系。
The relationship between protein synthesis and processes of cell division was studied by using synchronized cells of Bacillus subtilis 168. The addition of chloramphenicol at the beginning of synchronous growth prevented septum formation and cell division, suggesting the requirement of protein synthesis for the processes of cell division. Experiments in which the drug was added to the cells at different cell ages showed that the protein synthesis required for the initiation of septum formation was completed at about 15 min and that the protein synthesis required for cell division was completed at about 45 min. By interpreting the result from the concept of the transition point for protein synthesis, it was suggested that the processes of cell division in B. subtilis require at least two kinds of protein molecules which are synthesized at distinct stages in the cell cycle. This was supported by the result of an experiment in which starvation and the readdition of a required amino acid to exponentially growing cells induced two steps of synchronous cell division. Further, the two transition points are in agreement with the estimations obtained by residual division after the inhibition of protein synthesis in asynchronous cells. The relationship of the timing between the completion of chromosome replication and the two transition points was also studied.