Population diversity of Cistopus indicus inferred from mitochondrial DNA (Cytochrome b) variation from China and Vietnam
Population diversity of Cistopus indicus inferred from mitochondrial DNA (Cytochrome b) variation from China and Vietnam
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根据中国和越南线粒体 DNA（细胞色素 b）变异推断的 Cistopus indicus 种群多样性
DOI：
10.1080/13235818.2019.1688635
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发表时间：
2019-11-13
影响因子：
1
通讯作者：
Lu, Zhenming
中科院分区：
文献类型：
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作者：
Dou, Canfeng;Muhammad, Faiz;Lu, Zhenming
长蛸（Cistopus indicus）是中国头足类渔业的重要捕捞对象。它广泛分布于南太平洋和热带印度洋，从南海、菲律宾、马来西亚，到印度和巴基斯坦海域。我们从中国和越南的五个地点（舟山、温州、沙埕、湛江和芒街）采集了样本。从95个个体中扩增出了细胞色素b（Cytb）的675bp片段。共观察到27种单倍型和78个可变核苷酸位点。在所有种群中都观察到高单倍型多样性和低核苷酸多样性。系统发育分析将这些种群分为两个分支；一个由三个种群（舟山、温州和沙埕）组成，另一个由两个种群（湛江、芒街）组成。AMOVA分析检测到4.67%的遗传变异发生在种群内，95.33%发生在种群间。FST值介于0.014到0.993之间，凸显了种群间的高度遗传变异。假设Cytb基因的分子钟速率为2.15 - 2.6%/百万年，这两个分支可能在288 - 349万年前（上新世）分化。中性进化检验和错配分布分析表明近期种群扩张。目前的研究结果为该物种的遗传评估、管理和保护提供了有价值的信息。
ABSTRACT The octopus Cistopus indicus is an important target of cephalopod fisheries in China. It is widely distributed in the South Pacific and tropical Indian Ocean, from the South China Sea, the Philippines, Malaysia, to Indian and Pakistan seas. We collected specimens from five sites in China and Vietnam (Zhoushan, Wenzhou, Shacheng, Zhanjiang and Mangjie). A fragment of 675bp of cytochrome b (Cytb) was amplified from 95 individuals. A total of 27 haplotypes and 78 variable nucleotide sites was observed. High haplotype diversity and low nucleotide diversity were observed in all populations. The phylogenetic analysis separated these populations into two clades; one was composed of three populations (Zhoushan, Wenzhou and Shacheng), the other of two (Zhanjiang, Mangjie). AMOVA analysis detected that 4.67% of the genetic variation occurred within populations and 95.33% occurred among populations. FST values ranged from 0.014 to 0.993, highlighting the high genetic variation among the populations. Assuming a molecular clock with a rate of 2.15–2.6%/Ma for the Cytb gene, the two clades may have diverged 2.88–3.49 million years ago (Pliocene). Neutral evolution tests and mismatch distribution analysis suggested recent population expansion. The present results revealed valuable information for genetic assessment, management and conservation of this species.