Optogenetic Stimulation of MCH Neurons Increases Sleep
Optogenetic Stimulation of MCH Neurons Increases Sleep
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DOI：
10.1523/jneurosci.1225-13.2013
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发表时间：
2013-06-19
影响因子：
5.3
通讯作者：
Shiromani, Priyattam J.
中科院分区：
文献类型：
--
作者：
Konadhode, Roda Rani;Pelluru, Dheeraj;Shiromani, Priyattam J.
黑色素浓集激素（MCH）是一种存在于所有脊椎动物下丘脑的环状神经肽。MCH与许多行为有关，但缺乏直接证据。为了选择性地刺激MCH神经元，将光敏阳离子通道（channelrhodopsin-2）的基因插入野生型小鼠的MCH神经元中。3周后，每隔5 min刺激MCH神经元1 min，持续24 h。在夜晚开始时的10 Hz刺激加速了睡眠开始，减少了50%的清醒时间，增加了夜间非REM和REM睡眠的总时间，并增加了白天周期的睡眠强度。在所有唤醒神经元都活跃的昼夜节律时间的睡眠诱导表明MCH刺激可以有力地抵消唤醒神经元的组合唤醒促进信号。这可能对治疗失眠症有潜在的帮助。
Melanin concentrating hormone (MCH) is a cyclic neuropeptide present in the hypothalamus of all vertebrates. MCH is implicated in a number of behaviors but direct evidence is lacking. To selectively stimulate the MCH neurons the gene for the light-sensitive cation channel, channelrhodopsin-2, was inserted into the MCH neurons of wild-type mice. Three weeks later MCH neurons were stimulated for 1 min every 5 min for 24 h. A 10 Hz stimulation at the start of the night hastened sleep onset, reduced length of wake bouts by 50%, increased total time in non-REM and REM sleep at night, and increased sleep intensity during the day cycle. Sleep induction at a circadian time when all of the arousal neurons are active indicates that MCH stimulation can powerfully counteract the combined wake-promoting signal of the arousal neurons. This could be potentially useful in treatment of insomnia.