“De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry
“De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry
复制标题
通过纳电喷雾串联质谱法对凝胶内消化的蛋白质中回收的肽进行“从头”测序
DOI：
10.1385/mb:20:1:107
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发表时间：
2002
影响因子：
2.6
通讯作者：
M. Mann
中科院分区：
文献类型：
--
作者：
A. Shevchenko;I. Chernushevich;A. Shevchenko;M. Wilm;M. Mann
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
影响因子：
56.9
作者：
Lingner, J;Hughes, TR;Cech, TR
通讯作者：
Cech, TR
影响因子：
7.4
作者：
Shevchenko, A;Sunyaev, S;Standing, KG
通讯作者：
Standing, KG