Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum:: first observation of a reductive release mechanism during polyketide biosynthesis
Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum:: first observation of a reductive release mechanism during polyketide biosynthesis
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DOI：
10.1039/b708614h
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Skellam, Elizabeth
中科院分区：
文献类型：
--
作者：
Bailey, Andrew M.;Cox, Russell J.;Skellam, Elizabeth
从狭顶伞菌产Xenovulene培养物中分离到一个PKS基因，并对其进行了序列分析，结果表明该蛋白含有NT-、KS-、AT-、PT-、C-MET-和R-结构域，在米曲霉中的异源表达导致了3-甲基奥布林醛的产生，证明了末端还原酶域在产物释放中的作用。
Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures of Acremonium strictum indicated the presence of NT-, KS-, AT-, PT-, C-MeT- and R-domains; heterologous expression in Aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.