Assay for neural induction in the chick embryo.
Assay for neural induction in the chick embryo.
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DOI：
10.3791/1027
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发表时间：
2009-02-13
期刊：
影响因子：
--
通讯作者：
Finnell, Richard
中科院分区：
文献类型：
--
作者：
Psychoyos, Delphine;Finnell, Richard
鸡胚胎是研究早期胚胎发育的有价值的工具。它的透明度、可及性和易操作性，使其成为研究神经系统形成和初始模式的理想工具。这段视频演示了如何将组织器组织移植到宿主体内，这种方法是将亨森S节点(鸡胚中的组织器)移植到宿主有能力的外胚层上。组织者移植物通过神经诱导信号指示覆盖的幼稚组织采用神经命运。这种机制被称为神经诱导，构成了羊膜中脑和脊髓形成的第一步。这种方法主要用于鸡体内可能的神经诱导分子的表征。这段视频演示了神经诱导试验中的不同步骤；首先，唐纳的胚胎被移植并固定在盘子上。然后，宿主胚胎为新的培养做好准备。移植物被切除并移植到宿主区透明带的边缘。寄主培养18-22小时。该组件被固定和加工以供进一步应用(例如，原位杂交)。这种方法最初是由Waddington(1，2)和Gallera(3，4)设计的。
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host competent ectoderm. The organizer graft instructs overlying na ve tissue to adopt a neural fate via neural inducing signals. This mechanism is referred to as neural induction, and constitutes the initial step in the formation of brain and spinal cord in amniotes. This method is essentially used for the characterization of putative neural inducing molecules in chick. This video demonstrates the different steps in the assay for neural induction; First, the donnor embryo is explanted and pinned on a dish. Then, the host embryo is prepared for New culture. The graft is excised and transplanted to the host area pellucida margin. The host is cultured for 18-22 hrs. The assembly is fixed and processed for further applications (e.g. in situ hybridization). This method was originally devised by Waddington (1,2) and Gallera (3,4).