Reactive pocket epithelium in untreated chronic periodontal disease: possible derivation from developmental remnants of the enamel organ and root sheath
Reactive pocket epithelium in untreated chronic periodontal disease: possible derivation from developmental remnants of the enamel organ and root sheath
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DOI：
10.1034/j.1600-0714.2001.300308.x
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发表时间：
2001-03-01
影响因子：
3.3
通讯作者：
Gibbins, JR
中科院分区：
文献类型：
--
作者：
Hunter, N;Nicholls, B;Gibbins, JR
通过对未治疗的晚期牙周炎活检组织中中间丝蛋白表达的分析，研究了破坏性牙周炎的病理衬里上皮。细胞角蛋白（CK）对8/18简单的上皮细胞的特点是表达一致的分布模式局限于反应性口袋上皮。CK 8/18表达模式复杂，有两种明显的广泛表达。在三分之二的晚期疾病活检中，整个病理衬里上皮对CK 8和CK 18都有强烈反应。在其余部分中，更浅的衬里上皮混合有反应性和非反应性细胞灶，更深的上皮均匀反应。只有偶尔的高度局部反应的简单角蛋白（CK 8/18）被发现在衬里上皮活检从轻微发炎牙周组织。晚期牙周炎的病理性衬里上皮的进一步特征在于CK 14和CK 13的基底层中的α-表达，但不包括CK 4，其是复层鳞状上皮的基底上层的特征。细胞角蛋白17，高转换和迁移上皮细胞的标志物是极其可变的，表达模式与上皮或疾病状态的位置之间没有明确的关联。角化细胞典型的CK 10/11和间充质细胞特征性中间丝波形蛋白均无反应性。反应性衬里上皮的中间丝蛋白质谱与5例根尖周肉芽肿活检中3例存在的反应性上皮无法区分，其中3例活检含有来自Hertwig鞘（称为Malassez细胞剩余物）发育残留物激活的增生上皮。报告的数据是兼容的贡献由残余的发育上皮，包括减少釉质上皮和细胞休息的Malassez，反应性衬里上皮的龈下袋在慢性牙周炎的发病机制。
The pathological lining epithelium of destructive periodontitis was studied by analysis of the expression of intermediate filament proteins in biopsies of untreated advanced periodontitis. The cytokeratin (CK) pair 8/18 characteristic of simple epithelia was expressed consistently in a distribution pattern confined to the reactive pocket epithelium. The pattern of CK8/18 expression was complex with two broad presentations evident. In two-thirds of the advanced disease biopsies, the entire pathological lining epithelium was strongly reactive for both CK8 and CK18. In the remainder, the more superficial lining epithelium was mixed with foci of reactive and unreactive cells, with the deeper epithelium uniformly reactive. Only occasional highly localised reactivity for the simple keratins (CK8/18) was found in the lining epithelia of biopsies from minimally inflamed periodontal tissues. The pathological lining epithelium of advanced periodontitis was further characterised by the cc-expression in basal layers of CK14, and of CK13 but not CK4, which are characteristic of suprabasal layers of stratified squamous epithelia, Cytokeratin 17, a marker of high turnover and migrating epithelial cells was extremely variable with no clear association between expression pattern and location of the epithelium or disease status. There was no reactivity for CK10/11 typical of cornifying cells nor of vimentin, the characteristic intermediate filament of mesenchymal cells. The intermediate filament protein profile of the reactive lining epithelium was indistinguishable from the reactive epithelium present in three of five biopsies of periapical granulomas containing hyperplastic epithelium from activation of the developmental remnants of Hertwig's sheath, known as the cell rests of Malassez. The data reported are compatible with a contribution by remnants of developmental epithelium, including the reduced enamel epithelium and the cell rests of Malassez, to the reactive lining epithelium of the subgingival pocket in the pathogenesis of chronic periodontitis.