Cloning and sequencing of biofilm-associated protein (bapA) gene and its occurrence in different serotypes of Salmonella
Cloning and sequencing of biofilm-associated protein (bapA) gene and its occurrence in different serotypes of Salmonella
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DOI：
10.1111/j.1472-765x.2010.02975.x
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发表时间：
2011-02-01
影响因子：
2.4
通讯作者：
Singh, M.
中科院分区：
文献类型：
--
作者：
Biswas, R.;Agarwal, R. K.;Singh, M.
目的：沙门氏菌属具有在各种表面上形成生物膜的能力。沙门氏菌生物膜相关蛋白（Biofilm-associated protein，bapA）是沙门氏菌生物膜形成过程中的一个重要蛋白。本研究的目的是研究bapA基因在不同血清型沙门氏菌中的存在情况。方法和结果：采用专门设计的引物，对来自印度不同来源的34个血清型的67株沙门氏菌进行了bapA基因的筛选。所有菌株均产生阳性扩增，表明bapA基因在沙门氏菌属中是很保守的。用pGEM-T easy克隆载体将扩增的bapA基因片段克隆到大肠杆菌DH 5 α细胞中。部分序列分析表明，该基因编码667个碱基，对应218个氨基酸。从Salm菌株中获得该基因的部分序列分析。肠道感染与家禽来源的血清型以及其他动物/人畜共患病血清型密切相关。研究的意义和影响：BapA基因可作为不同来源的该微生物的属特异性检测的靶点。bapA蛋白的抗原性指数表明其具有保护和诊断的潜力。
Aims:Salmonella spp. has the capability to form biofilm on various surfaces. Biofilm-associated protein (bapA), a large surface protein has been shown to play a leading role in the development of biofilm in Salmonella. Objective of this study was to investigate the presence of bapA gene in different serotypes of Salmonella spp. and to characterize DNA fragment encoding bapA protein of Salmonella Enteritidis.Methods and Results:Sixty-seven Salmonella strains belonging to 34 serovars isolated from diverse sources in India were screened for the presence of bapA gene employing a primer designed for the purpose. All the strains yielded a positive amplification indicating that the bapA gene is well conserved in Salmonella spp. The amplified gene fragment of bapA was cloned in Escherichia coli (DH5 alpha) cells by using pGEM-T easy cloning vector. On partial sequence analysis, the product exhibited 667 base pairs, corresponding to 218 amino acids.ConclusionsBapA gene was found to be highly conserved in Salmonella. Partial sequence analysis of this gene from a strain of Salm. Enteritidis revealed close association with serotypes of poultry origin and also with some other animal/zoonotic serotypes.Significance and Impact of the Study:BapA gene can be targeted for the genus-specific detection of this organism from different sources. Antigenic index of bapA protein indicates its protective and diagnostic potentials.