Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples.
Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples.
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DOI：
10.1016/j.xpro.2022.101203
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Powell RLR
中科院分区：
文献类型：
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作者：
Yang X;Fox A;Powell RLR
从先前感染SARS-CoV-2或接种COVID-19疫苗的人获得的乳汁中的抗体可能为母乳喂养的婴儿提供被动免疫。尽管母乳中的抗体对公共卫生具有重要意义，但很少有检测方法被建立起来。在本方案中，我们描述了一种优化的间接ELISA测定法，旨在测量人乳中的SARS-CoV-2反应性抗体，该方法可用于未稀释样品的快速筛选，或将样品指定为相对低，中等或高滴度。有关本方案使用和执行的完整详细信息，请参阅。用于检测抗刺突抗体或抗其他免疫原抗体的人乳中SARS-CoV-2反应性抗体的测定方案可用作未稀释乳汁的筛查工具终点滴度测定可指定反应的相对效力从先前感染SARS-CoV-2或接种COVID-19疫苗的乳汁中获得的抗体可为母乳喂养的婴儿提供被动免疫。尽管母乳中的抗体对公共卫生具有重要意义，但很少有检测方法被建立起来。在本方案中，我们描述了一种优化的间接ELISA测定法，旨在测量人乳中的SARS-CoV-2反应性抗体，该方法可用于未稀释样品的快速筛选，或将样品指定为相对低，中等或高滴度。
Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to. A protocol for measuring SARS-CoV-2-reactive antibodies in human milk Used to detect anti-Spike antibodies or those against other immunogens Can be used as a screening tool on undiluted milk Endpoint titer determination allows designation of relative potency of response Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer.