Hypoalphalipoproteinemia: postprandial response of subjects with preprandial normotriglyceridemia and hypertriglyceridemia to various diets.
Hypoalphalipoproteinemia: postprandial response of subjects with preprandial normotriglyceridemia and hypertriglyceridemia to various diets.
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低α脂蛋白血症：餐前正常甘油三酯血症和高甘油三酯血症受试者对各种饮食的餐后反应。
DOI：
10.1016/0026-0495(93)90043-n
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Scanu,AM
中科院分区：
文献类型：
--
作者：
Edelstein,C;Fredenrich,C;Schuelke,JC;Jensen,WE;Sitrin,M;Iverius,PH;Scanu,AM
在4名原发性低脂蛋白血症（HP）男性患者和4名年龄性别匹配的对照组中研究了富含饱和（SFA）或多不饱和（[PUFA] ω-6或ω-3）脂肪酸的单次口服脂肪餐（60 g脂肪/m2体表面积）对餐后脂蛋白水平的影响。膳食中包含维生素A，以标记具有棕榈酸视黄酯（RP）的来源于尿液的富含维生素A的颗粒（TRP）。HP受试者为轻度高脂血症（A组）或正常脂血症（B组），并对肝素后脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（HTGL）活性和载脂蛋白（apo）E亚型进行表型分析。在餐后24小时内评估餐后总血浆甘油三酯（TG）、高密度脂蛋白（HDL）胆固醇、乳糜微粒（Sf> 1，000）和非乳糜微粒（Sf> 1，000）组分中的RP和TG浓度。在每个时间点，还测量了HDL组成和大小以及载脂蛋白分布。SFA餐后，HP受试者在8小时（对照组为4小时）时血浆TG水平达到最大值，在12 - 24小时（对照组为8 - 12小时）缓慢恢复至基线水平。与此相反，ω-6餐后，A组受试者的血浆TG水平下降，而B组受试者和对照组仅显示出小幅升高。ω-7餐后的结果介于SFA和ω-3餐之间。与B组相比，A组受试者的RP相关TRP水平较高，清除率较慢，空腹LPL活性降低30%至50%，空腹血浆载脂蛋白C-III水平升高1.5倍至2倍。HP受试者的主要餐前HDL亚类是HDL 3，其显示出SFA餐后胆固醇酯（CE）相对降低和TG水平升高。ω-3餐后，A组受试者的HDL显示TG降低，CE反向升高，磷脂（PL）和游离胆固醇（FC）水平无变化或变化较小。结果表明，轻度餐前高脂血症的HP受试者对单次脂肪餐的反应不同于正常脂血症的受试者，这种差异是HP加上其他因素（如低LPL和HTGL活性、高血浆载脂蛋白C-III水平和载脂蛋白E2表型）的结果。
The effect of a single oral fat meal (60 g fat/m2body surface area) enriched in either saturated (SFA) or polyunsaturated ([PUFA] ω-6 or ω-3) fatty acids on postprandial lipoprotein levels was studied in four men with primary hypoalphalipoproteinemia (HP) and in four-age- and sex-matched controls. Vitamin A was included in the meal to label intestinally derived triglyceride-rich particles (TRP) with retinyl palmitate (RP). The HP subjects were either mildly hypertriglyceridemic (group A) or normotriglyceridemic (group B) and were phenotyped for post-heparin lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) activities and apolipoprotein (apo) E isoforms. Postprandial total plasma triglyceride (TG), high-density lipoprotein (HDL) cholesterol, and RP and TG concentrations in the chylomicron (Sf> 1,000) and nonchylomicron (Sf> 1,000) fractions were evaluated for 24 hours after the meal. At each time point, HDL composition and size and apolipoprotein distributions were also measured. Following the SFA meal, HP subjects had maximal plasma TG levels at 8 hours (4 hours in controls) with a slow return to baseline levels at 12 to 24 hours (8 to 12 hours for controls). In contrast, after the ω-6 meal plasma TG levels decreased in group A subjects, while group B subjects and controls showed only a small increase. The results after the ω-7 meal were intermediate between the SFA and the ω-3 meal. When compared with group B, subjects in group A showed higher levels of RP-associated TRP, slower clearance rates, 30% to 50% lower fasting LPL activity, and 1.5-fold to twofold higher fasting plasma apo C-III levels. The major preprandial HDL subclass in HP subjects was HDL3, which showed a relative decrease in cholesterol esters (CE) and an increase in TG levels following the SFA meal. After the ω-3 meal, HDL of group A subjects showed a decrease in TG, a reciprocal increase in CE, and either no changes or minor changes in phospholipid (PL) and free-cholesterol (FC) levels. The results show that HP subjects with mild preprandial hypertriglyceridemia respond to a single fat meal differently than subjects with normotriglyceridemia, and that this difference is the result of HP in addition to other factors such as low LPL and HTGL activities, high plasma apo C-III levels, and apo E2 phenotype.